Determination of optimal glycerol concentration for optical tissue clearing

Eungjun Youn; Taeyoon Son; Han-Sung Kim; Byungjo Jung

doi:10.1117/12.909790

9 February 2012 Determination of optimal glycerol concentration for optical tissue clearing

Eungjun Youn, Taeyoon Son, Han-Sung Kim, Byungjo Jung

Proceedings Volume 8207, Photonic Therapeutics and Diagnostics VIII; 82070J (2012) https://doi.org/10.1117/12.909790
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

The laser scattering in tissue is significant in diagnostic and therapeutic purposes of laser. Many studies have been conducted to minimize laser scattering in tissue and therefore, to maximize the clinical efficacy by enhancing photon density. Optical clearing agents (OCAs) have been employed for optical tissue clearing (OTC). This study was aimed to investigate the optimal concentration of an OCA, glycerol, in topical application,, so that it can be utilized for clinical diagnosis and therapy in dermatology. Glycerol was topically applied to avoid possible edema caused by dermal injection. The effect of OTC was quantitatively evaluated as a function of the concentration of glycerol with various methods. Optical methods such as optical coherence tomography (OCT) and an integrating sphere were used to assess the enhancement of light penetration depth and refractive index matching. In addition, a non-optical method, ultrasound scanner, was utilized to evaluate quantitatively collagen dissociation. The results revealed that 70 % glycerol was the optimal concentration of OTC for topical application. This study may provide a guideline regarding to the use of glycerol for optimal diagnostic and therapeutic effects in dermatology.

Citation Download Citation

Eungjun Youn, Taeyoon Son, Han-Sung Kim, and Byungjo Jung "Determination of optimal glycerol concentration for optical tissue clearing", Proc. SPIE 8207, Photonic Therapeutics and Diagnostics VIII, 82070J (9 February 2012); https://doi.org/10.1117/12.909790

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available