Ray-tracing study on the post-scanner variable beam expansion optics in a two-photon microscopy system

Do-Hyun Kim; Cristin Welle; Victor Krauthamer

doi:10.1117/12.909144

9 February 2012 Ray-tracing study on the post-scanner variable beam expansion optics in a two-photon microscopy system

Do-Hyun Kim, Cristin Welle, Victor Krauthamer

Proceedings Volume 8226, Multiphoton Microscopy in the Biomedical Sciences XII; 822610 (2012) https://doi.org/10.1117/12.909144
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Due to the low signal levels typical of two-photon microscopy (TPM) in biological samples, optical design optimization is critical. One of the most important factors is overfilling of the back aperture of the objective lens. A variable beam expander is commonly placed before the scanning mirrors to achieve this goal, however, this may cause degradation of image quality due to increased dispersion. Additionally, scanning mirror size restricts the degree of expansion, which often prevents the overfilling of objective lens back aperture. We investigated the implementation of variable beam expansion optics after the scanning mirrors. Ray-tracing analyses confirmed that the post-scanner beam expansion has two key advantages over the conventional pre-scanner beam expansion approach: decreasing the number of optical elements reduces pulse dispersion and reducing the size of the scanning mirror enables faster scanning. Resolution and aberration of a TPM with post-scanner beam expansion optics were analysed.

Citation Download Citation

Do-Hyun Kim, Cristin Welle, and Victor Krauthamer "Ray-tracing study on the post-scanner variable beam expansion optics in a two-photon microscopy system", Proc. SPIE 8226, Multiphoton Microscopy in the Biomedical Sciences XII, 822610 (9 February 2012); https://doi.org/10.1117/12.909144

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available