Performance validation of EMCCD and ICCD based near-infrared fluorescence imaging systems on a fluorescence solid phantom

Banghe Zhu; Eva M. Sevick-Muraca

doi:10.1117/12.908954

2 February 2012 Performance validation of EMCCD and ICCD based near-infrared fluorescence imaging systems on a fluorescence solid phantom

Banghe Zhu, Eva M. Sevick-Muraca

Author Affiliations +

Proceedings Volume 8229, Optical Diagnostics and Sensing XII: Toward Point-of-Care Diagnostics; and Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurement of Tissue IV; 822911 (2012) https://doi.org/10.1117/12.908954
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Near infrared (NIR) fluorescence imaging has been successfully applied for non-invasive assessment of both lymphatic architecture and function as well as potential disease markers of lymphatic dysfunction in clinical studies with intradermal injection of indocyanine green (ICG). For new "first-in-humans" NIR fluorescence imaging agents that need to be employed at far lower quantities, NIR fluorescence imaging devices with high measurement sensitivity are most favorable. However, the measurement sensitivity of NIR fluorescence imaging devices is limited by various parameters, including quantum efficiency of CCD chip, noise sources in the CCD camera, and the leakage of excitation light through optical filters. In this contribution, we present a quantum dot-based fluorescence solid phantom and its use for characterization of excitation light leakage and measurement sensitivity in both the intensified CCD (ICCD) and Electron Multiplying CCD (EMCCD) based NIR fluorescence imaging devices. The stability of the constructed quantum dot-based fluorescence solid phantom was first demonstrated and used to demonstrate higher measurement sensitivity compared of the ICCD as opposed to the EMCCD based NIR fluorescence imaging device when integration time were maintained less than 1.0 s. The phantom was used to assess the calculated transmission ratio, R, to minimize noise owing to excitation light leakage and show optimized filtering capabilities. The constructed quantum dot based solid phantom and the methodology for measuring parameters of transmission ratio and SNR can be used as a standard and quantifiable metric for installation and operational qualification of all NIR fluorescence imaging devices.

Citation Download Citation

Banghe Zhu and Eva M. Sevick-Muraca "Performance validation of EMCCD and ICCD based near-infrared fluorescence imaging systems on a fluorescence solid phantom", Proc. SPIE 8229, Optical Diagnostics and Sensing XII: Toward Point-of-Care Diagnostics; and Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurement of Tissue IV, 822911 (2 February 2012); https://doi.org/10.1117/12.908954

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KEYWORDS

Luminescence

Near infrared

Imaging systems

Electron multiplying charge coupled devices

Solids

Signal to noise ratio

CCD cameras

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