A portable microfluidic-based biophotonic sensor for extracellular H2O2 measurements

V. Koman; G. Suárez; Ch. Santschi; V. J. Cadarso; J. Brugger; N. von Moos; V. I. Slaveykova; O. J. F. Martin

doi:10.1117/12.2008329

22 March 2013 A portable microfluidic-based biophotonic sensor for extracellular H₂O₂ measurements

V. Koman, G. Suárez, Ch. Santschi, V. J. Cadarso, J. Brugger, N. von Moos, V. I. Slaveykova, O. J. F. Martin

Proceedings Volume 8572, Advanced Biomedical and Clinical Diagnostic Systems XI; 857218 (2013) https://doi.org/10.1117/12.2008329
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

In this work a portable analytical biosensor for real-time extracellular monitoring of released hydrogen peroxide (H₂O₂ ) is presented. The biosensor is based on the optical detection of the cytochrome c (cyt c) oxidation state. The setup consists of an integrated microscope combined with a compact spectrometer. The light being absorbed by cyt c is enhanced via multiscattering produced by random aggregates of polystyrene beads in a cross-linked cyt c matrix. Using ink-jet printing technique, the sensing elements, namely cyt c loaded polystyrene aggregates, are fabricated with high reliability in terms of repeatability of size and sensitivity. Additionally, the sensing elements are enclosed in a microfluidic channel assuring a fast and efficient analytes delivery. As an example, the effect of trace concentrations of functionalized cadmium selenide/zinc sulfide (CdSe/ZnS) core shell quantum dots on the green algae Chlamydomonas reinhardtii is investigated, showing extracellular H₂O₂ release with different production rates over a period of 1 hour. In conclusion, the presented portable biosensor enables the highly sensitive and non-invasive real-time monitoring of the cell metabolism of C. reinhardtii.

Citation Download Citation

V. Koman, G. Suárez, Ch. Santschi, V. J. Cadarso, J. Brugger, N. von Moos, V. I. Slaveykova, and O. J. F. Martin "A portable microfluidic-based biophotonic sensor for extracellular H₂O₂ measurements", Proc. SPIE 8572, Advanced Biomedical and Clinical Diagnostic Systems XI, 857218 (22 March 2013); https://doi.org/10.1117/12.2008329

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