Ultra-deep imaging of turbid samples by enhanced photon harvesting

Viera Crosignani; Alexander Dvornikov; Enrico Gratton

doi:10.1117/12.2002101

22 February 2013 Ultra-deep imaging of turbid samples by enhanced photon harvesting

Viera Crosignani, Alexander Dvornikov, Enrico Gratton

Proceedings Volume 8588, Multiphoton Microscopy in the Biomedical Sciences XIII; 858810 (2013) https://doi.org/10.1117/12.2002101
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

We constructed an advanced detection system for two-photon fluorescence microscopy that allows us to image in biological tissue and tissue phantoms up to the depth of a few mm with micron resolution. The innovation lies in the detection system which is much more sensitive to low level fluorescence signals than the fluorescence detection configuration used in conventional two-photon fluorescence microscopes. A wide area photocathode photomultiplier tube (PMT) was used to detect fluorescence photons directly from a wide (1 inch diameter) area of the turbid sample, as opposed to the photon collection by the microscope objective which can only collect light from a relatively small area of the sample. The optical path between the sample and the photocathode is refractive index matched to curtail losses at the boundaries due to reflections. The system has been successfully employed in the imaging of tissue phantoms simulating brain optical properties and in biological tissues, such as murine small intestine, colon, tumors, and other samples. The system has in-depth fluorescence lifetime imaging (FLIM) capabilities and is also highly suitable for SHG signal detection, such as collagen fibers and muscles, due to the intrinsically forward-directed propagation of SHG photons.

Citation Download Citation

Viera Crosignani, Alexander Dvornikov, and Enrico Gratton "Ultra-deep imaging of turbid samples by enhanced photon harvesting", Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 858810 (22 February 2013); https://doi.org/10.1117/12.2002101

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