Assay for optical determination of biogenic amines using microtiterplates

Polona Nedeljko; Matejka Turel; Aleksandra Lobnik

doi:10.1117/12.2019486

3 May 2013 Assay for optical determination of biogenic amines using microtiterplates

Polona Nedeljko, Matejka Turel, Aleksandra Lobnik

Proceedings Volume 8774, Optical Sensors 2013; 87741A (2013) https://doi.org/10.1117/12.2019486
Event: SPIE Optics + Optoelectronics, 2013, Prague, Czech Republic

Abstract

Direct determination of catecholamine noradreanaline (NOR) is presented using o-phthaldialdehyde (OPA) as an indicator reagent. The fluorescent assay in which OPA forms with NOR a fluorescent complex (OPA-NOR) can be monitored at neutral, physiological conditions (pH 7) and performed in microtiterplates. The determination of NOR is optimal in the concentration range from 4.0×10^-7 to 1.0×10^-5 M and limit of detection is 4.0×10^-7 M. The OPA-NOR complex maximum intensity is reached within 5 minutes. Dopamine and adrenaline could not be determined using the same approach.

Citation Download Citation

Polona Nedeljko, Matejka Turel, and Aleksandra Lobnik "Assay for optical determination of biogenic amines using microtiterplates ", Proc. SPIE 8774, Optical Sensors 2013, 87741A (3 May 2013); https://doi.org/10.1117/12.2019486

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