A simple model for deep tissue attenuation correction and large organ analysis of Cerenkov luminescence imaging

Frezghi Habte; Arutselvan Natarajan; David S. Paik; Sanjiv Sam Gambhir

doi:10.1117/12.2043879

19 March 2014 A simple model for deep tissue attenuation correction and large organ analysis of Cerenkov luminescence imaging

Frezghi Habte, Arutselvan Natarajan, David S. Paik, Sanjiv Sam Gambhir

Author Affiliations +

Proceedings Volume 9033, Medical Imaging 2014: Physics of Medical Imaging; 90334H (2014) https://doi.org/10.1117/12.2043879
Event: SPIE Medical Imaging, 2014, San Diego, California, United States

Abstract

Cerenkov luminescence imaging (CLI) is an emerging cost effective modality that uses conventional small animal optical imaging systems and clinically available radionuclide probes for light emission. CLI has shown good correlation with PET for organs of high uptake such as kidney, spleen, thymus and subcutaneous tumors in mouse models. However, CLI has limitations for deep tissue quantitative imaging since the blue-weighted spectral characteristics of Cerenkov radiation attenuates highly by mammalian tissue. Large organs such as the liver have also shown higher signal due to the contribution of emission of light from a greater thickness of tissue. In this study, we developed a simple model that estimates the effective tissue attenuation coefficient in order to correct the CLI signal intensity with a priori estimated depth and thickness of specific organs. We used several thin slices of ham to build a phantom with realistic attenuation. We placed radionuclide sources inside the phantom at different tissue depths and imaged it using an IVIS Spectrum (Perkin-Elmer, Waltham, MA, USA) and Inveon microPET (Preclinical Solutions Siemens, Knoxville, TN). We also performed CLI and PET of mouse models and applied the proposed attenuation model to correct CLI measurements. Using calibration factors obtained from phantom study that converts the corrected CLI measurements to %ID/g, we obtained an average difference of less that 10% for spleen and less than 35% for liver compared to conventional PET measurements. Hence, the proposed model has a capability of correcting the CLI signal to provide comparable measurements with PET data.

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Frezghi Habte, Arutselvan Natarajan, David S. Paik, and Sanjiv Sam Gambhir "A simple model for deep tissue attenuation correction and large organ analysis of Cerenkov luminescence imaging", Proc. SPIE 9033, Medical Imaging 2014: Physics of Medical Imaging, 90334H (19 March 2014); https://doi.org/10.1117/12.2043879

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KEYWORDS

Positron emission tomography

Signal attenuation

Tissues

Tissue optics

Spleen

Imaging systems

Liver

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