Study optical properties of biological tissue in the presence of microbubbles

Homa Assadi; Vincent Lee; Raffi Karshafian; Alexandre Douplik

doi:10.1117/12.2075673

5 March 2015 Study optical properties of biological tissue in the presence of microbubbles

Homa Assadi, Vincent Lee, Raffi Karshafian, Alexandre Douplik

Proceedings Volume 9321, Optical Interactions with Tissue and Cells XXVI; 93210X (2015) https://doi.org/10.1117/12.2075673
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Optical contrast agents introduce distinct features to induce detectable changes in native tissue properties [1]. In ultrasound imaging, microbubbles (MBs) – a gas-core shell-encapsulated agent – are used clinically as contrast agents. The working hypothesis of this study is that microbubbles can be employed as an intravascular contrast agent in optical imaging systems. Microbubbles can produce a refractive index mismatch which makes it distinguishable from surrounding media. In this work, the interaction of collimated light and microbubbles in a [1] biological phantom solution was investigated. The biological medium was comprised of intralipid and human blood which was constructed to cover the range of soft tissue optical properties. The effect of microbubbles on the optical properties such as reduced scattering and absorption coefficients were considered. Diffuse reflectance (DR) and total transmittance (TT) of a biological phantom solution were measured using a spectroscopic integrating sphere system in the absence and presence of Definity® microbubbles. The optical properties were computed using the inverse adding doubling (IAD) software. The presence of microbubbles increased DR and decreased TT of the phantom. In the presence of MB’s (2.5% volume concentration), the reflectance of the phantom increased by 25% in the optical window. There is no absorption event and only scattering happened after light-microbubbles interactions. The reduced scattering coefficient increased significantly (30%) indicating the potential use of MBs as optical contrast agents. In conclusion, reflectance of a media can be enhanced by adding microbubbles to increase scattering properties and more light was detected returning to the surface of tissue.

Citation Download Citation

Homa Assadi, Vincent Lee, Raffi Karshafian, and Alexandre Douplik "Study optical properties of biological tissue in the presence of microbubbles", Proc. SPIE 9321, Optical Interactions with Tissue and Cells XXVI, 93210X (5 March 2015); https://doi.org/10.1117/12.2075673

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
11 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Blood

Tissue optics

Scattering

Light scattering

Optical properties

Diffuse reflectance spectroscopy

Transmittance

Show All Keywords

Keywords/Phrases

Search In:

Publication Years