Reduction of phase volume error in off-axis quantitative phase microscopy using optimum phase-shift

Mohammad Reza Jafarfard; Behnam Tayebi; Razie Jalali Nasab; Dug Young Kim

doi:10.1117/12.2078681

10 March 2015 Reduction of phase volume error in off-axis quantitative phase microscopy using optimum phase-shift

Mohammad Reza Jafarfard, Behnam Tayebi, Razie Jalali Nasab, Dug Young Kim

Proceedings Volume 9386, Practical Holography XXIX: Materials and Applications; 93860P (2015) https://doi.org/10.1117/12.2078681
Event: SPIE OPTO, 2015, San Francisco, California, United States

Abstract

We present a method to reduce the inherent errors caused by band-pass filter in off-axis quantitative phase microscopy and propose the optimum condition that can minimize these errors. We found that phase information of a sample in frequency domain nonlinearly oscillates as a function of the phase-shift correspond to the sample and its medium and the phase information of a sample inside the band-pass filter can be maximized by a proper phase-shift. Through numerical simulations and actual experiments, we demonstrate that the error in phase volume measurement can be effectively reduced by the enhancement of phase signal inside band-pass region using an optimum amount of phase that can be controlled by either changing medium index or wavelength of illumination.

Citation Download Citation

Mohammad Reza Jafarfard, Behnam Tayebi, Razie Jalali Nasab, and Dug Young Kim "Reduction of phase volume error in off-axis quantitative phase microscopy using optimum phase-shift ", Proc. SPIE 9386, Practical Holography XXIX: Materials and Applications, 93860P (10 March 2015); https://doi.org/10.1117/12.2078681

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