Quantitative label-free sperm imaging by means of transport of intensity

Praveen Kumar Poola; Vimal Prabhu Pandiyan; Varshini Jayaraman; Renu John

doi:10.1117/12.2211608

9 March 2016 Quantitative label-free sperm imaging by means of transport of intensity

Praveen Kumar Poola, Vimal Prabhu Pandiyan, Varshini Jayaraman, Renu John

Author Affiliations +

Proceedings Volume 9718, Quantitative Phase Imaging II; 97180O (2016) https://doi.org/10.1117/12.2211608
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Most living cells are optically transparent which makes it difficult to visualize them under bright field microscopy. Use of contrast agents or markers and staining procedures are often followed to observe these cells. However, most of these staining agents are toxic and not applicable for live cell imaging. In the last decade, quantitative phase imaging has become an indispensable tool for morphological characterization of the phase objects without any markers. In this paper, we report noninterferometric quantitative phase imaging of live sperm cells by solving transport of intensity equations with recorded intensity measurements along optical axis on a commercial bright field microscope.

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Citation Download Citation

Praveen Kumar Poola, Vimal Prabhu Pandiyan, Varshini Jayaraman, and Renu John "Quantitative label-free sperm imaging by means of transport of intensity", Proc. SPIE 9718, Quantitative Phase Imaging II, 97180O (9 March 2016); https://doi.org/10.1117/12.2211608

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