Phase-contrast tomography of neuronal tissues: from laboratory- to high resolution synchrotron CT

Mareike Töpperwien; Martin Krenkel; Kristin Müller; Tim Salditt

doi:10.1117/12.2238496

3 October 2016 Phase-contrast tomography of neuronal tissues: from laboratory- to high resolution synchrotron CT

Mareike Töpperwien, Martin Krenkel, Kristin Müller, Tim Salditt

Proceedings Volume 9967, Developments in X-Ray Tomography X; 99670T (2016) https://doi.org/10.1117/12.2238496
Event: SPIE Optical Engineering + Applications, 2016, San Diego, California, United States

Abstract

Assessing the three-dimensional architecture of neuronal tissues with sub-cellular resolution presents a significant analytical challenge. Overcoming the limitations associated with serial slicing, phase-contrast x-ray tomography has the potential to contribute to this goal. Even compact laboratory CT at an optimized liquid-metal jet micro- focus source combined with suitable phase-retrieval algorithms and preparation protocols can yield renderings with single cell sensitivity in millimeter sized brain areas of mouse. Here, we show the capabilities of the setup by imaging a Golgi-Cox impregnated mouse brain. Towards higher resolution we extend these studies at our recently upgraded waveguide-based cone-beam holo-tomography instrument GINIX at DESY. This setup allows high resolution recordings with adjustable field of view and resolution, down to the voxel sizes in the range of a few ten nanometers. The recent results make us confident that important issues of neuronal connectivity can be addressed by these methods, and that 3D (virtual) histology with nanoscale resolution will become an attractive modality for neuroscience research.

Conference Presentation

Citation Download Citation

Mareike Töpperwien, Martin Krenkel, Kristin Müller, and Tim Salditt "Phase-contrast tomography of neuronal tissues: from laboratory- to high resolution synchrotron CT", Proc. SPIE 9967, Developments in X-Ray Tomography X, 99670T (3 October 2016); https://doi.org/10.1117/12.2238496

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