Optical properties of synthetic and natural melanin: effects of heat and oxidation

Michael L. Denton; Lorenzo Brancaleon; Gary Noojin; Anvay Patil; Saranshu Singla; Ali Dhinojwala

doi:10.1117/12.2609033

7 February 2023 Optical properties of synthetic and natural melanin: effects of heat and oxidation

Michael L. Denton, Lorenzo Brancaleon, Gary Noojin, Anvay Patil, Saranshu Singla, Ali Dhinojwala

Proceedings Volume PC11958, Optical Interactions with Tissue and Cells XXXIII; and Advanced Photonics in Urology; PC1195809 (2023) https://doi.org/10.1117/12.2609033
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Several experimental results have indicated interesting chemical properties of melanosome particles (MPs) derived from retinal pigment epithelial (RPE) cells. These properties may lead to changes in optical properties (absorption in particular) as well. Here, we show characterizations of the MPs before and after various perturbations, including temperature elevation, chemical oxidation, and laser exposure. Methods of analysis included protein fixation, fluorescence, fluorescence anisotropy, dynamic light scattering, transmitted electron microscopy, and confocal microscopy. Finally, we show chemical differences between isolated MPs and those in cytoplasm.

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Michael L. Denton, Lorenzo Brancaleon, Gary Noojin, Anvay Patil, Saranshu Singla, and Ali Dhinojwala "Optical properties of synthetic and natural melanin: effects of heat and oxidation", Proc. SPIE PC11958, Optical Interactions with Tissue and Cells XXXIII; and Advanced Photonics in Urology, PC1195809 (7 February 2023); https://doi.org/10.1117/12.2609033

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KEYWORDS

Optical properties

Chemical lasers

Confocal fluorescent microscopy

Confocal microscopy

Dichroic materials

Fluorescence anisotropy

Luminescence

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