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2 March 2022 Label-free cargo localization iSCAT microscopy uncovers dynamic transport of intracellular vesicles and underlying active cytoskeletal network
Jin-Sung Park, Il-Buem Lee, Hyeon-Min Moon, Seok-Cheol Hong, Minhaeng Cho
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Proceedings Volume PC11972, Label-free Biomedical Imaging and Sensing (LBIS) 2022; PC119720R (2022) https://doi.org/10.1117/12.2610448
Event: SPIE BiOS, 2022, San Francisco, California, United States
Abstract
We introduce the cargo-localization iSCAT microscopy, label-free, live-cell imaging technique, that visualizes the traffic of cargos in a massively parallel fashion and hence uncovers the actively-used cytoskeletal network from cargos’ traces. By applying a particle localization algorithm to over a hundred cargos moving in a lamellipodium, the fine architecture of the cytoskeletal highway could be reconstructed. We also discuss a host of interesting kinetic events associated with cargo transport such as intermittent pausing, turnover, and frequent jamming of cargos due to physical collision with other obstacles or at the intersection of the highway.
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Jin-Sung Park, Il-Buem Lee, Hyeon-Min Moon, Seok-Cheol Hong, and Minhaeng Cho "Label-free cargo localization iSCAT microscopy uncovers dynamic transport of intracellular vesicles and underlying active cytoskeletal network", Proc. SPIE PC11972, Label-free Biomedical Imaging and Sensing (LBIS) 2022, PC119720R (2 March 2022); https://doi.org/10.1117/12.2610448
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KEYWORDS
Microscopy
Environmental sensing
Functional imaging
Image resolution
Luminescence
Particles
Reconstruction algorithms
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