Cell migration is an essential cellular process that help cells to develop complex organisms, organs, and tissues, arrange cells into specialized architecture and help organize the nervous system. Nanoscale imaging has the potential to provide new insight into the mechanics of cell migration. However, quantification over time in nanoscale imaging remains difficult. Here, we visualize cell migration by non-bleaching nanoscale imaging. We present a set of quantitative metrics - length, branching, gaps, distribution, and curvature - to quantitatively analyze cell migration at the nanoscale over time.
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