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16 March 2023 Fewer photons for fast FLIM using analog multiplexing of laser clock (Conference Presentation)
Rishyashring R. Iyer, Janet E. Sorrells, Geng Wang, Lingxiao Yang, Haohua Tu, Stephen A. Boppart
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Proceedings Volume PC12390, High-Speed Biomedical Imaging and Spectroscopy VIII; PC123900S (2023) https://doi.org/10.1117/12.2649178
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
It is challenging to image sparse samples with fast FLIM since the laser clock is not directly sampled. Instead, it is inferred from the cumulative photon statistics of an entire line. We present a method for registering the photon arrival times to the excitation using time-domain multiplexing of the laser clock to ensure accuracy for fewer photons for fast FLIM. Our technique also does not add to the existing bottleneck of data throughput and eliminates the errors in registering the photons from different frames, yielding more accurate images at faster frame rates.
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Rishyashring R. Iyer, Janet E. Sorrells, Geng Wang, Lingxiao Yang, Haohua Tu, and Stephen A. Boppart "Fewer photons for fast FLIM using analog multiplexing of laser clock (Conference Presentation)", Proc. SPIE PC12390, High-Speed Biomedical Imaging and Spectroscopy VIII, PC123900S (16 March 2023); https://doi.org/10.1117/12.2649178
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KEYWORDS
Fluorescence lifetime imaging
Photons
Clocks
Multiplexing
Analog electronics
Electronics
Microscopy
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