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16 March 2023 Platelet micro-aggregate diagnostics with quantitative phase microscopy (Conference Presentation)
Oliver Hayden, Christian Klenk, Johanna Erber, David Fresacher, Stefan Röhrl, Manuel Lengl, Dominik Heim, Hedwig Irl, Martin Schlegel, Tobias Lahmer, Klaus Diepold, Sebastian Rasch, Bernhard Haller
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Proceedings Volume PC12391, Label-free Biomedical Imaging and Sensing (LBIS) 2023; PC123910I (2023) https://doi.org/10.1117/12.2668846
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
We discuss label-free high-throughput phase imaging, allowing the identification of platelet-platelet microaggregates and their components at single-platelet resolution in COVID-19 patients. The sample preparation-free measurement can be performed in 2 minutes with a benchtop-sized flow cytometer from a diluted blood sample matching requirement for point-of-care-testing in the ICU. Most importantly, the microfluidic solution allows for low shear stress to minimize loss of microaggregates yet achieving high throughput. We validated a positive correlation of (1) increased platelet-platelet concentration, (2) composition, and (3) size distribution with severity of COVID-19. Most importantly, the microaggregates we observe are in fact in a size regime of a single leukocyte, which allows for a sufficiently long circulation time in the peripheral blood system for quantification.
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Oliver Hayden, Christian Klenk, Johanna Erber, David Fresacher, Stefan Röhrl, Manuel Lengl, Dominik Heim, Hedwig Irl, Martin Schlegel, Tobias Lahmer, Klaus Diepold, Sebastian Rasch, and Bernhard Haller "Platelet micro-aggregate diagnostics with quantitative phase microscopy (Conference Presentation)", Proc. SPIE PC12391, Label-free Biomedical Imaging and Sensing (LBIS) 2023, PC123910I (16 March 2023); https://doi.org/10.1117/12.2668846
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KEYWORDS
Diagnostics
Microscopy
Biological samples
Blood
COVID 19
Image resolution
Microfluidics
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