Optical microfluidic droplet calorimeter

Joerg Martini; Ignacio López-Peña; Qiushu Chen; Anne Plochowietz; Daniel Cohen

doi:10.1117/12.3003881

13 March 2024 Optical microfluidic droplet calorimeter

Joerg Martini, Ignacio López-Peña, Qiushu Chen, Anne Plochowietz, Daniel Cohen

Proceedings Volume PC12837, Microfluidics, BioMEMS, and Medical Microsystems XXII; PC128370A (2024) https://doi.org/10.1117/12.3003881
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Calorimetry is a label-free technique to study molecular interaction and measure their enthalpy changes in solution. We have developed a microfluidic calorimetry platform that uses optical means to measure these enthalpy changes in sub-nanoliter aqueous droplets. Thermochromic liquid crystals act as optical transducers to convert temperature changes caused by the reaction heat to a shift in their reflectance spectrum. We implemented large area fiber coupled LED illumination optics and multi spot detection along the microfluidic channel to measure the temperature changes in moving microfluidic droplets. To calibrate the temperature response of our system we implemented localized laser heating of individual droplets at 1464nm and monitored the heat dissipation of those droplets in the microfluidic channel. We showed that we can observe milli-Kelvin temperature changes in droplets with millisecond time resolution.

Conference Presentation

Citation Download Citation

Joerg Martini, Ignacio López-Peña, Qiushu Chen, Anne Plochowietz, and Daniel Cohen "Optical microfluidic droplet calorimeter", Proc. SPIE PC12837, Microfluidics, BioMEMS, and Medical Microsystems XXII, PC128370A (13 March 2024); https://doi.org/10.1117/12.3003881

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