Melanoma is the most serious kind of skin cancer. Circulating melanoma cells, the prognosis marker for metastasis, are present in the circulation at the early stage. Thus, quantitative detection of rare circulating melanoma cells is essential for monitoring tumor metastasis and prognosis evaluation. Compared with in vitro assays, in vivo flow cytometry is able to identify circulating tumor cells without drawing blood. Here, we have built in vivo photoacoustic flow cytometry based on the high absorption coefficient and monitoring circulating melanoma cells, which is useful for evaluating cancer stage and monitoring therapeutic effects.
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