Untangled Brillouin light scattering for mechanical imaging in turbid samples

Giuseppe Antonacci; Renzo Vanna; Dario Polli; Cristian Manzoni; Giulio Cerullo

doi:10.1117/12.3002718

13 March 2024 Untangled Brillouin light scattering for mechanical imaging in turbid samples

Giuseppe Antonacci, Renzo Vanna, Dario Polli, Cristian Manzoni, Giulio Cerullo

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Proceedings Volume PC12844, Optical Elastography and Tissue Biomechanics XI; PC128440F (2024) https://doi.org/10.1117/12.3002718
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Brillouin microscopy is a label-free and noncontact optical method to measure fundamental viscoelastic properties of biological systems. One of the main challenges in Brillouin microscopy is given by the strong Rayleigh background light overcoming the Brillouin peaks that are spectrally located at a few GHz from the laser frequency. Here, we demonstrate a common-path and broadband notch filter providing an unprecedented 65 dB extinction ratio. We demonstrate the filter by acquiring Brillouin spectra of highly turbid samples, such as pure milk and unprocessed vertebra bone tissues.

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Citation Download Citation

Giuseppe Antonacci, Renzo Vanna, Dario Polli, Cristian Manzoni, and Giulio Cerullo "Untangled Brillouin light scattering for mechanical imaging in turbid samples", Proc. SPIE PC12844, Optical Elastography and Tissue Biomechanics XI, PC128440F (13 March 2024); https://doi.org/10.1117/12.3002718

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KEYWORDS

Turbidity

Light scattering

Optical filters

Tunable filters

Biological imaging

Biomedical optics

Image filtering

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