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13 March 2024 Digital holography with embedded microlasers: a preliminary study
Peter Naglič, Zuhail P. Kottoli, Matjaž Humar
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Proceedings Volume PC12846, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXII; PC1284609 (2024) https://doi.org/10.1117/12.3001423
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
In this study, we investigate the use of microlasers as light sources for digital holographic microscopy embedded in the sample. Microlasers are 50-μm sized dye-doped self-assembled cholesteric liquid-crystal microdroplets that isotropically emit single-mode laser light. By employing an epi-illumination configuration of a standard optical microscope, we excited a single microlaser beneath the sample plane and subsequently acquired in-line holograms of various samples placed between the microlaser and microscope objective. Embedding the light source enabled us to uniquely acquire in-line digital holograms in transmission even though the sample is observed in an epi-illumination configuration and could in principle be infinitely thick on one side.
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Peter Naglič, Zuhail P. Kottoli, and Matjaž Humar "Digital holography with embedded microlasers: a preliminary study", Proc. SPIE PC12846, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXII, PC1284609 (13 March 2024); https://doi.org/10.1117/12.3001423
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KEYWORDS
Digital holography
Holograms
Reconstruction algorithms
3D image reconstruction
Dyes
Light sources and illumination
Microscopes
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