STORM imaging buffer at immersion oil refractive index

Youngseop Lee; Minchol Lee; Yeunho Lee; Dongwoo Kim; Hongrae Kim; Donghoon Koo; Kangwon Lee; Jeongmin Kim

doi:10.1117/12.3002140

13 March 2024 STORM imaging buffer at immersion oil refractive index

Youngseop Lee, Minchol Lee, Yeunho Lee, Dongwoo Kim, Hongrae Kim, Donghoon Koo, Kangwon Lee, Jeongmin Kim

Proceedings Volume PC12849, Single Molecule Spectroscopy and Superresolution Imaging XVII; PC1284906 (2024) https://doi.org/10.1117/12.3002140
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Stochastic optical reconstruction microscopy (STORM) achieves super-resolution imaging by blinking individual dye molecules in thiol-containing media. While STORM is well-established for imaging thin biological specimens, its application to thick tissues has been limited by light scattering. Mounting media with an oil refractive index have shown promise in improving imaging depth and resolution in optical microscopy, but such buffers have not been explored for STORM. Here, we present a 3-pyridinemethanol-based STORM buffer with a refractive index matching standard immersion oil. Our buffer demonstrates comparable performance to conventional STORM buffers and exceptional stability for 5 weeks, enabling imaging of numerous cells on a single slide and larger field-of-view imaging. With perfect index matching, our buffer simplifies imaging and holds potential for lightsheet STORM applications in thick tissues.

Conference Presentation

Citation Download Citation

Youngseop Lee, Minchol Lee, Yeunho Lee, Dongwoo Kim, Hongrae Kim, Donghoon Koo, Kangwon Lee, and Jeongmin Kim "STORM imaging buffer at immersion oil refractive index", Proc. SPIE PC12849, Single Molecule Spectroscopy and Superresolution Imaging XVII, PC1284906 (13 March 2024); https://doi.org/10.1117/12.3002140

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