Immunothrombosis of acute care patients quantified with phase imaging flow cytometry

Oliver Hayden; Klaus Diepold; Christian Klenk; Johanna Erber M.D.; David Fresacher; Stefan Röhrl; Manuel Lengl; Dominik Heim; Hedwig Irl; Martin Schlegel; Bernhard Haller; Tobias Lahmer; Sebastian Rasch; Johannes Krell; Kerem Delikoyun; Qianyu Chen; Win Sen Kuan; Matt Cove; Gerhard Schneider; John Song; Chew Ka Lip; Komal Javarappa

doi:10.1117/12.3022776

13 March 2024 Immunothrombosis of acute care patients quantified with phase imaging flow cytometry

Oliver Hayden, Klaus Diepold, Christian Klenk, Johanna Erber M.D., David Fresacher, Stefan Röhrl, Manuel Lengl, Dominik Heim, Hedwig Irl, Martin Schlegel, Bernhard Haller, Tobias Lahmer, Sebastian Rasch, Johannes Krell, Kerem Delikoyun, Qianyu Chen, Win Sen Kuan, Matt Cove, Gerhard Schneider, John Song, Chew Ka Lip, Komal Javarappa

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Proceedings Volume PC12852, Quantitative Phase Imaging X; PC128520I (2024) https://doi.org/10.1117/12.3022776
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Immunothrombosis is a critical aspect affecting patients in acute care settings, especially in conditions involving infection, trauma, or severe inflammation. The interplay between hemostasis, innate immunity, and inflammation becomes highly relevant in these scenarios and can significantly impact patient outcomes. Blood cell aggregates are potential functional cellular biomarkers for prognostic and predictive biomarkers of immunothrombosis but require, due to the low logistical stability of the aggregates, a point-of-care (POC) solution for standardizable diagnostics. Here, we present a POC-compatible method that combines a quantitative phase imaging method with a microfluidic chip and a customized image analysis, resulting in a label- and sample preparation-free high-throughput imaging flow cytometer. We optimized our conditions to mimic the flow conditions in vessels at a low shear stress of ~1.000 s−1. In the acute care uses case, we demonstrate the dynamics of blood cell aggregates in critical care patients and discuss the potential impact on clinical workflows.

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Oliver Hayden, Klaus Diepold, Christian Klenk, Johanna Erber M.D., David Fresacher, Stefan Röhrl, Manuel Lengl, Dominik Heim, Hedwig Irl, Martin Schlegel, Bernhard Haller, Tobias Lahmer, Sebastian Rasch, Johannes Krell, Kerem Delikoyun, Qianyu Chen, Win Sen Kuan, Matt Cove, Gerhard Schneider, John Song, Chew Ka Lip, and Komal Javarappa "Immunothrombosis of acute care patients quantified with phase imaging flow cytometry", Proc. SPIE PC12852, Quantitative Phase Imaging X, PC128520I (13 March 2024); https://doi.org/10.1117/12.3022776

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KEYWORDS

Flow cytometry

Phase imaging

COVID 19

Imaging systems

Biological imaging

Blood

Image analysis

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