Super-resolution chemical imaging based on absorption-induced photothermal relaxation localization

Delong Zhang

doi:10.1117/12.3003680

13 March 2024 Super-resolution chemical imaging based on absorption-induced photothermal relaxation localization

Delong Zhang

Proceedings Volume PC12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024; PC1285506 (2024) https://doi.org/10.1117/12.3003680
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Super-resolution imaging has facilitated groundbreaking discoveries by surpassing classical limitations. However, the commonly employed fluorescence-based approach exhibits inherent drawbacks, such as low labeling efficiency and potential toxicity. To address these challenges, we propose photothermal relaxation localization (PEARL) microscopy, a broadly applicable label-free chemical imaging technique that exploits the unique temporal characteristics of sub-diffraction limit objects. These features can be extracted through higher harmonic demodulation, effectively transcending the diffraction limit. Unlike existing methods, PEARL demonstrates a highly linear power dependence, enabling enhanced signal levels without the need for high peak power. Furthermore, PEARL's applicability extends to virtually any absorptive material. Experimental validations encompassing a range of materials, from gold nanoparticles to subcellular droplets, corroborate PEARL's ability to achieve superior resolution. In summary, PEARL introduces a versatile and potent optical super-resolution imaging technique with the potential to catalyze advancements across diverse scientific domains.

Conference Presentation

Citation Download Citation

Delong Zhang "Super-resolution chemical imaging based on absorption-induced photothermal relaxation localization", Proc. SPIE PC12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024, PC1285506 (13 March 2024); https://doi.org/10.1117/12.3003680

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KEYWORDS

Super resolution

Imaging spectroscopy

Demodulation

Diffraction limit

Feature extraction

Gold nanoparticles

Microscopy

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