Imaging through translucide layers in digital holographic microscopy and application to the metrology of human red blood cells observed behind an endothelial cell layer

Jérôme Dohet-Eraly; Jonathan Nasimba Nacimba; Frank Dubois

doi:10.1117/12.3022549

18 June 2024 Imaging through translucide layers in digital holographic microscopy and application to the metrology of human red blood cells observed behind an endothelial cell layer

Jérôme Dohet-Eraly, Jonathan Nasimba Nacimba, Frank Dubois

Author Affiliations +

Proceedings Volume PC12996, Unconventional Optical Imaging IV; PC129960H (2024) https://doi.org/10.1117/12.3022549
Event: SPIE Photonics Europe, 2024, Strasbourg, France

Abstract

Imaging and measuring moving objects behind translucide layers is of high interest in numerous applications, in various fields. We developed in this study a method using digital holographic microscopy for that purpose, with a partial spatial coherence illumination. The presented study includes numeric simulations for the assessment of the measurement accuracy, as a function of both the object and the translucide layer parameters. Experiments are performed with the in vitro analysis of human red blood cells moving behind an edothelial cell layer. Such a configuration mimics the real blood vessel, which is of crucial importance for a better understanding of the behaviour of the blood cells in blood circulation.

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Jérôme Dohet-Eraly, Jonathan Nasimba Nacimba, and Frank Dubois "Imaging through translucide layers in digital holographic microscopy and application to the metrology of human red blood cells observed behind an endothelial cell layer", Proc. SPIE PC12996, Unconventional Optical Imaging IV, PC129960H (18 June 2024); https://doi.org/10.1117/12.3022549

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