CONFERENCE PROCEEDINGS
Advanced Search
Home > Journals > J. Biomed. Opt. > Volume 12 > Issue 2 > Article
Open Access
1 March 2007 Fluorescence spectroscopy and birefringence of molecular changes in maturing rat tail tendon
Renee M. Korol, Helen M. Finlay, Melanie J. Josseau, Alexandra R. Lucas, Peter B. Canham
Author Affiliations +
Journal of Biomedical Optics, Vol. 12, Issue 2, 024011 (March 2007). https://doi.org/10.1117/1.2714055
Abstract
Tissue remodeling during maturation, wound healing, and response to vascular stress involves molecular changes of collagen and elastin in the extracellular matrix (ECM). Two optical techniques are effective for investigating these changes—laser-induced fluorescence (LIF) spectroscopy and polarizing microscopy. LIF spectroscopy integrates the signal from both elastin and collagen cross-linked structure, whereas birefringence is a measure of only collagen. Our purpose is (1) to evaluate the rat tail tendon (RTT) spectroscopy against data from purified extracted protein standards and (2) to correlate the two optical techniques in the study of RTT and skin. Spectra from tissue samples from 27 male rats and from extracted elastin and collagen were obtained using LIF spectroscopy (357 nm). Birefringence was measured on 5-μm histological sections of the same tissue. Morphometric analysis reveals that elastin represents approximately 10% of tendon volume and contributes to RTT fluorescence. RTT maximum fluorescence emission intensity (FEImax), which includes collagen and elastin, increases with animal weight (R2=0.64). Birefringence, when plotted against weight, increases to a plateau (nonlinear correlation: R2=0.90), tendon having greater birefringence than skin. LIF spectroscopy and collagen fiber birefringence are shown to provide complementary measurements of molecular structure (tendon birefringence versus FEImax at R2=0.60).
©(2007) Society of Photo-Optical Instrumentation Engineers (SPIE)
Citation Download Citation
Renee M. Korol, Helen M. Finlay, Melanie J. Josseau, Alexandra R. Lucas, and Peter B. Canham "Fluorescence spectroscopy and birefringence of molecular changes in maturing rat tail tendon," Journal of Biomedical Optics 12(2), 024011 (1 March 2007). https://doi.org/10.1117/1.2714055
Published: 1 March 2007
JOURNAL ARTICLE
 8 PAGES
DOWNLOAD PAPER SAVE TO MY LIBRARY
GET CITATION
Lens.org Logo
CITATIONS
Cited by 42 scholarly publications.
Advertisement
Advertisement
RIGHTS & PERMISSIONS
Get copyright permission  Get copyright permission on Copyright Marketplace
KEYWORDS
Collagen
Birefringence
Skin
Tissues
Luminescence
Proteins
Fluorescence spectroscopy
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top