Multiphoton adaptation of a commercial low-cost confocal microscope for live tissue imaging

James J. Mancuso; Adam M. Larson; Theodore G. Wensel; Peter Saggau

doi:10.1117/1.3139850

1 May 2009 Multiphoton adaptation of a commercial low-cost confocal microscope for live tissue imaging

James J. Mancuso, Adam M. Larson, Theodore G. Wensel, Peter Saggau

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Journal of Biomedical Optics, Vol. 14, Issue 3, 034048 (May 2009). https://doi.org/10.1117/1.3139850

Abstract

The Nikon C1 confocal laser scanning microscope is a relatively inexpensive and user-friendly instrument. We describe a straightforward method to convert the C1 for multiphoton microscopy utilizing direct coupling of a femtosecond near-infrared laser into the scan head and fiber optic transmission of emission light to the three-channel detector box. Our adapted system can be rapidly switched between confocal and multiphoton mode, requires no modification to the original system, and uses only a few custom-made parts. The entire system, including scan mirrors and detector box, remain under the control of the user-friendly Nikon EZ-C1 software without modification.

©(2009) Society of Photo-Optical Instrumentation Engineers (SPIE)

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James J. Mancuso, Adam M. Larson, Theodore G. Wensel, and Peter Saggau "Multiphoton adaptation of a commercial low-cost confocal microscope for live tissue imaging," Journal of Biomedical Optics 14(3), 034048 (1 May 2009). https://doi.org/10.1117/1.3139850

Published: 1 May 2009

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