Probing cell surface interactions using atomic force microscope cantilevers functionalized for quantum dot-enabled Förster resonance energy transfer

Zhe Sun; Ameet Juriani; Gerald A. Meininger; Kenith E. Meissner

doi:10.1117/1.3174429

1 July 2009 Probing cell surface interactions using atomic force microscope cantilevers functionalized for quantum dot-enabled Förster resonance energy transfer

Zhe Sun, Ameet Juriani, Gerald A. Meininger, Kenith E. Meissner

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Journal of Biomedical Optics, Vol. 14, Issue 4, 040502 (July 2009). https://doi.org/10.1117/1.3174429

Abstract

Förster resonance energy transfer (FRET) between quantum dot (QD) donors and red fluorescent protein (RFP)-tagged integrin acceptors in live cells is reported for the first time. A silica microsphere was coated with CdSe/ZnS QDs and mounted to the cantilever of an atomic force microscope (AFM). The QD microsphere is then conjugated with fibronectin to bind with RFP-αv integrins expressed on the surface of HeLa cells. Following AFM-controlled cell contact with the QD-microsphere structure, FRET is observed between the QD-RFP pair using a photobleaching measurement technique. This FRET probe technique provides a novel tool for studying the cell surface receptor-ligand interactions in biomedical and biological research.

©(2009) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Zhe Sun, Ameet Juriani, Gerald A. Meininger, and Kenith E. Meissner "Probing cell surface interactions using atomic force microscope cantilevers functionalized for quantum dot-enabled Förster resonance energy transfer," Journal of Biomedical Optics 14(4), 040502 (1 July 2009). https://doi.org/10.1117/1.3174429

Published: 1 July 2009

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