Imaging of goblet cells as a marker for intestinal metaplasia of the stomach by one-photon and two-photon fluorescence endomicroscopy

Hongchun Bao; Alex Boussioutas; Jeremy Reynolds; Sarah Russell; Min Gu

doi:10.1117/1.3269681

1 November 2009 Imaging of goblet cells as a marker for intestinal metaplasia of the stomach by one-photon and two-photon fluorescence endomicroscopy

Hongchun Bao, Alex Boussioutas, Jeremy Reynolds, Sarah Russell, Min Gu

Author Affiliations +

Journal of Biomedical Optics, Vol. 14, Issue 6, 064031 (November 2009). https://doi.org/10.1117/1.3269681

Abstract

Goblet cells are a requirement for the diagnosis of intestinal metaplasia of the stomach. The gastric mucosa is lined by a monolayer of columnar epithelium with some specialization at the crypts, but there are no goblet cells in normal gastric epithelium. The appearance of goblet cells in gastric epithelium is an indicator of potential malignant progression toward adenocarcinoma. Therefore, in vivo three-dimensional imaging of goblet cells is essential for diagnoses of a premalignant stage of gastric cancers called intestinal metaplasia. We used mouse intestine, which has goblet cells, as a model of intestinal metaplasia. One-photon confocal fluorescence endomicroscopy and two-photon fluorescence endomicroscopy are employed for 3-D imaging of goblet cells. The penetration depth, the sectioning ability, and the photobleaching information of imaging are demonstrated. Both endomicroscopy techniques can three-dimensionally observe goblet cells in mouse large intestine and achieve an imaging depth of 176 µm. The two-photon fluorescence endomicroscopy shows higher resolution and contrast of the imaging sections at each depth. In addition, two-photon fluorescence endomicroscopy also has advantages of sectioning ability and less photobleaching. These results prove that two-photon fluorescence endomicroscopy is advantageous in diagnoses of a premalignant stage of gastric cancers.

©(2009) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Hongchun Bao, Alex Boussioutas, Jeremy Reynolds, Sarah Russell, and Min Gu "Imaging of goblet cells as a marker for intestinal metaplasia of the stomach by one-photon and two-photon fluorescence endomicroscopy," Journal of Biomedical Optics 14(6), 064031 (1 November 2009). https://doi.org/10.1117/1.3269681

Published: 1 November 2009

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KEYWORDS

Luminescence

Intestine

Endomicroscopy

Cancer

Stomach

Stereoscopy

Diagnostics

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