Ratiometric spectral imaging for fast tumor detection and chemotherapy monitoring in vivo

Jae-Youn Hwang; Daniel L. Farkas; Lali K. Medina-Kauwe; Zeev Gross; Harry B. Gray

doi:10.1117/1.3589299

1 June 2011 Ratiometric spectral imaging for fast tumor detection and chemotherapy monitoring in vivo

Jae-Youn Hwang, Daniel L. Farkas, Lali K. Medina-Kauwe, Zeev Gross, Harry B. Gray

Author Affiliations +

Funded by: NIH

Journal of Biomedical Optics, Vol. 16, Issue 6, 066007 (June 2011). https://doi.org/10.1117/1.3589299

Abstract

We report a novel in vivo spectral imaging approach to cancer detection and chemotherapy assessment. We describe and characterize a ratiometric spectral imaging and analysis method and evaluate its performance for tumor detection and delineation by quantitatively monitoring the specific accumulation of targeted gallium corrole (HerGa) into HER2-positive (HER2 +) breast tumors. HerGa temporal accumulation in nude mice bearing HER2 + breast tumors was monitored comparatively by a. this new ratiometric imaging and analysis method; b. established (reflectance and fluorescence) spectral imaging; c. more commonly used fluorescence intensity imaging. We also tested the feasibility of HerGa imaging in vivo using the ratiometric spectral imaging method for tumor detection and delineation. Our results show that the new method not only provides better quantitative information than typical spectral imaging, but also better specificity than standard fluorescence intensity imaging, thus allowing enhanced in vivo outlining of tumors and dynamic, quantitative monitoring of targeted chemotherapy agent accumulation into them.

©(2011) Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation Download Citation

Jae-Youn Hwang, Daniel L. Farkas, Lali K. Medina-Kauwe, Zeev Gross, and Harry B. Gray "Ratiometric spectral imaging for fast tumor detection and chemotherapy monitoring in vivo," Journal of Biomedical Optics 16(6), 066007 (1 June 2011). https://doi.org/10.1117/1.3589299

Published: 1 June 2011

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
7 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

CITATIONS

Cited by 18 scholarly publications and 4 patents.

Explore citations on Lens.org

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Tumors

Imaging spectroscopy

Luminescence

In vivo imaging

Image classification

Molecules

Breast

Show All Keywords

RELATED CONTENT

Noninvasive imaging of absolute PpIX concentration distribution in nonmelanoma skin tumors...
Proceedings of SPIE (March 13 2013)

Multimode optical imaging for translational chemotherapy in vivo tumor...
Proceedings of SPIE (February 28 2011)

Spectral imaging detects breast cancer in fresh unstained specimens
Proceedings of SPIE (February 22 2006)

Liposomes As Carriers Of Hydrophobic Photosensitizers In Vivo Increased...
Proceedings of SPIE (February 19 1988)

Fluorescence depolarization of normal and diseased skin tissues
Proceedings of SPIE (April 16 1998)

Microscopic and mesoscopic spectral bioimaging
Proceedings of SPIE (May 10 1996)

Subscribe to Digital Library

Receive Erratum Email Alert

Show All Keywords

Keywords/Phrases

Search In:

Publication Years