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12 August 2013 Blind unmixing of spectrally resolved lifetime images
Farzad Fereidouni, Gerhard A. Blab, Hans C. Gerritsen
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Journal of Biomedical Optics, Vol. 18, Issue 8, 086006 (August 2013). https://doi.org/10.1117/1.JBO.18.8.086006
Abstract
A method, is presented for blind unmixing spectrally resolved fluorescence lifetime images. The method is based on the combined analysis of spectral and lifetime phasors and allows unmixing of up to three components without any prior knowledge. Fractional intensities, spectra and decay curves of the individual components can be extracted with this new technique. The reliability and sensitivity are investigated and the possibility of extending the method to unmix more components is discussed. The method is evaluated on mixtures of fluorescent dyes and labeled cells.
© 2013 Society of Photo-Optical Instrumentation Engineers (SPIE) 0091-3286/2013/$25.00 © 2013 SPIE
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Farzad Fereidouni, Gerhard A. Blab, and Hans C. Gerritsen "Blind unmixing of spectrally resolved lifetime images," Journal of Biomedical Optics 18(8), 086006 (12 August 2013). https://doi.org/10.1117/1.JBO.18.8.086006
Published: 12 August 2013
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Cited by 13 scholarly publications.
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KEYWORDS
Image segmentation
Luminescence
Clouds
Visualization
Binary data
RGB color model
Superposition
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