Open Access
6 February 2014 Photosensitizer fluorescence and singlet oxygen luminescence as dosimetric predictors of topical 5-aminolevulinic acid photodynamic therapy induced clinical erythema
Srivalleesha Mallidi, Sriram R. Anbil, Seonkyung Lee, Dieter Manstein, Stefan A. Elrington, Garuna Kositratna, David Schoenfeld, Brian W. Pogue, Steven J. Davis, Tayyaba Hasan
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Abstract
The need for patient-specific photodynamic therapy (PDT) in dermatologic and oncologic applications has triggered several studies that explore the utility of surrogate parameters as predictive reporters of treatment outcome. Although photosensitizer (PS) fluorescence, a widely used parameter, can be viewed as emission from several fluorescent states of the PS (e.g., minimally aggregated and monomeric), we suggest that singlet oxygen luminescence (SOL) indicates only the active PS component responsible for the PDT. Here, the ability of discrete PS fluorescence-based metrics (absolute and percent PS photobleaching and PS re-accumulation post-PDT) to predict the clinical phototoxic response (erythema) resulting from 5-aminolevulinic acid PDT was compared with discrete SOL (DSOL)-based metrics (DSOL counts pre-PDT and change in DSOL counts pre/post-PDT) in healthy human skin. Receiver operating characteristic curve (ROC) analyses demonstrated that absolute fluorescence photobleaching metric (AFPM) exhibited the highest area under the curve (AUC) of all tested parameters, including DSOL based metrics. The combination of dose-metrics did not yield better AUC than AFPM alone. Although sophisticated real-time SOL measurements may improve the clinical utility of SOL-based dosimetry, discrete PS fluorescence-based metrics are easy to implement, and our results suggest that AFPM may sufficiently predict the PDT outcomes and identify treatment nonresponders with high specificity in clinical contexts.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
Srivalleesha Mallidi, Sriram R. Anbil, Seonkyung Lee, Dieter Manstein, Stefan A. Elrington, Garuna Kositratna, David Schoenfeld, Brian W. Pogue, Steven J. Davis, and Tayyaba Hasan "Photosensitizer fluorescence and singlet oxygen luminescence as dosimetric predictors of topical 5-aminolevulinic acid photodynamic therapy induced clinical erythema," Journal of Biomedical Optics 19(2), 028001 (6 February 2014). https://doi.org/10.1117/1.JBO.19.2.028001
Published: 6 February 2014
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CITATIONS
Cited by 46 scholarly publications.
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KEYWORDS
Luminescence

Photodynamic therapy

Picosecond phenomena

Oxygen

Skin

Statistical analysis

Photography

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