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28 May 2015 Role of 5-aminolevulinic acid-conjugated gold nanoparticles for photodynamic therapy of cancer
Zhenxi Zhang, Sijia Wang, Hao Xu, Bo Wang, Cuiping Yao
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Journal of Biomedical Optics, Vol. 20, Issue 5, 051043 (May 2015). https://doi.org/10.1117/1.JBO.20.5.051043
Abstract
There are three possible mechanisms for 5-aminolevulinic acid (5-ALA) conjugated gold nanoparticles (GNPs) through electrostatic bonding for photodynamic therapy (PDT) of cancer: GNPs delivery function, singlet oxygen generation (SOG) by GNPs irradiated by light, and surface resonance enhancement (SRE) of SOG. Figuring out the exact mechanism is important for further clinical treatment. 5-ALA-GNPs and human chronic myeloid leukemia K562 cells were used to study delivery function and SOG by GNPs. The SRE of SOG enabled by GNPs was explored by protoporphyrin IX (PpIX)-GNPs conjugate through electrostatic bonding. Cell experiments show that the GNPs can improve the efficiency of PDT, which is due to the vehicle effect of GNPs. PpIX–GNPs conjugate experiments demonstrated that SOG can be improved about 2.5 times over PpIX alone. The experiments and theoretical results show that the local field enhancement (LFE) via localized surface plasmon resonance (LSPR) of GNPs is the major role; the LFE was dependent on the irradiation wavelength and the GNP’s size. The LFE increased with an increase of the GNP size (2R ≤50  nm). However, the LSPR function of the GNPs was not found in cell experiments. Our study shows that in 5-ALA-conjugated GNPs PDT, the delivery function of GNPs is the major role.
© 2015 Society of Photo-Optical Instrumentation Engineers (SPIE) 1083-3668/2015/$25.00 © 2015 SPIE
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Zhenxi Zhang, Sijia Wang, Hao Xu, Bo Wang, and Cuiping Yao "Role of 5-aminolevulinic acid-conjugated gold nanoparticles for photodynamic therapy of cancer," Journal of Biomedical Optics 20(5), 051043 (28 May 2015). https://doi.org/10.1117/1.JBO.20.5.051043
Published: 28 May 2015
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KEYWORDS
Photodynamic therapy
Oxygen
Nanoparticles
Gold
Luminescence
Picosecond phenomena
Absorption
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