We looked into three distinct topologies, using a one-dimensional photonic crystal with a defect layer in two symmetrical and one asymmetrical configurations as an optical biosensor application for detection in tuberculosis (TB). In order to investigate the optical transmission characteristics of such structures, we used a well-known transfer matrix approach. A shift in resonant peak is estimated, when blood of healthy or TB infected human is infiltrated in defect layer. The impact of several parameters, including the thickness of the defect layer, the number of unit cells, and the angle of incidence for all three configurations, has been studied. The sensitivity, quality factor, figure of merit, and detection limit for all structures have been studied and it is found that asymmetric structure is the best suitable structure for optical sensor application in the present case.