Laser-induced breakdown spectroscopy (LIBS) is a technique developed in the last few decades for simultaneous multi-element characterization of various materials. Multiplexed detection of analytes is particularly useful in the realm of food contaminant detection, where the contaminant can be one or a combination of adulterants. Paper-based assays are an emerging platform for food-contaminant detection. However, most paper-based assays do not perform multiplexed detection. For food contaminant outbreak prevention and remediation, rapid multiplexed detection could make a difference in response speed. This study applies LIBS to the concept of multi-analyte detection on paper-based bioassays. In the envisioned bioassay, a variety of analytes are labeled with unique lanthanides, a technique common to the well-established field of mass cytometry. The presence of single or multiple lanthanide labels indicates the presence of single or multiple types of contaminants. We aim to implement LIBS for multiplexed detection of lanthanide labels. To investigate data analysis approaches for multi-lanthanide detection, we evaluate univariate data analysis and spectral unmixing approaches on samples containing combinations of europium, dysprosium, gadolinium, praseodymium, and neodymium. We find that the intense signal generated by Eu, matrix effects, selfabsorption, and spectral overlap affect the outcome of the results. Future studies will continue the investigation to identify the most appropriate approach.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.