Cancer has become a public health problem worldwide. Radiotherapy may be a treatment to a number of types of cancer,
frequently using gamma-radiation with sources such as 137Cs and 60Co, with varying doses, dose rates, and exposure
times to obtain a better as a stimulant for cell proliferation and tissue healing process. However, its effects on cancer
cells are not yet well elucidated. The purpose of this work was to evaluate the effects of the LPL on breast cancer
cultures after ionizing radiation. The breast cancer-MDA-MB-231 cells were gamma irradiated by a 60Co source, with
dose of 2.5 Gy. After 24h, cells were submitted to LPL irradiation using a red laser emitting at λ= 660 nm, with output
power of 40 mW and exposure time of 30 s and 60 s. The plates were uniformly irradiated, with energy of 1.2 J and
2.4 J, respectively. Cell viability was analyzed using the exclusion method with trypan blue. Our results show that breast
cancer cells submitted to LPL after ionizing radiation remained 95 % viable. No statistically significant differences were
observed between laser and control untreated cells, (P > 0.05). These findings suggest that LPL did not influenced cancer
cells viability.
Optical properties of the biological tissue play an important role to a correct use of optical techniques for therapy and diagnosis. The mice skin presents morphological differences due to characteristics such as gender, body mass and age. Murine models are frequently used in pre-clinical trials in optical therapy and diagnosis. Therefore, the assessment of the skin tissue in animal models is needed for a proper understanding of how light interacts with skin. Noninvasive techniques such as optical coherence tomography (OCT) have been used to obtain optical information of the tissue, as the attenuation coefficient, with the advantage of obtaining sectional images in real time. In this study, eight female BALB/c albino mice (twenty-four weeks old) and eight male C57BL/6 black mice (eight weeks old) were used to measure the attenuation coefficient of the light in the skin, utilizing the OCT technique, aiming to check for influence of the aging process. Two moments were assessed twenty-two weeks apart from each other. Our data show that the aging process significantly affects the light attenuation coefficient in mice skin. Twenty-two weeks after, statistical significant differences were observed between groups within a same strain. We conclude that light attenuation coefficient of mice skin may be influenced by factors such as disorganization of the dermis. Morphological aspects of skin should be taken into account in studies that involve optical strategies in murine models.
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