The results of quantum dot (QD) probe preparation for multiplexed single-cell array staining and analysis are reported. By controlling the reaction temperature, time, and ratio of Cd to Se, multicolor CdSe QDs emitting fluorescence ranging from purple to red in a safer, simpler, and more convenient way than traditional methods is obtained. To detect cells using these oil-soluble QDs, they are first coated with water-soluble thioglycolic aid (TGA) so that biocompatible multiwavelength bioprobes can be obtained. QDs’ surface is somewhat damaged when binding TGA to QDs is found, which results in a reduction of QDs’ emission wavelength and a slight blue shift of QDs’ emission wavelength after water-soluble modification with TGA. Comparison of the emission spectrum showed that it is negligible, and the fluorescent properties of QDs capped by TGA are still satisfactory. Living cells are then stained with multiplexed probes by conjugating TGA-QDs with antibodies specific to these cell antigens. Changes in fluorescence intensity can indicate change in the relative quantity of antigens expressed in the same cell caused by external stimulus, offering effective methods to multiplexed optical analysis of single cells.
To meet the requirement of the space applications, digital thrusters with larger scale units should be employed, which means that the dimension of each thruster unit should be further reduced. In this paper, the system function structure for the satellite attitude control is introduced and a micro arrayed thruster is designed to improve the ignition properties in tiny chambers. A four layers structure of the micro thruster unit is presented and the ignition resistor is studied in detail. The structural mechanics analyzing results and the fabrication sample are presented. As an important part, the solid propellant is analyzed and the prescription is presented. To improve the uniformity of the propellant loading, a special device is developed by which the propellant can be once loaded into the array. To test the performance of the thruster, we developed an optical measurement system based on a pendulum structure. The system principle is introduced and a series of mathematical model was established. The impulse measurement system based on laser interference is presented. By this system, the impulse property of the micro thruster based on the solid propellant is studied and the test results are analyzed.
The measurement of the wheel-aligning parameters is very important in the production of vehicles. In this paper, we present a non-contact measuring technique for the inspection of wheel-aligning quality in which the laser triangulation is employed based on structural light sensors. First, the main purpose of the inspection is introduced with corresponding parameters illustrated. Meanwhile, the main principles of the system and the structural light sensors are analyzed in detail. In the paper, the image processing techniques are studied, which include the noise eliminating algorithms and the symbol extraction methods. The image quality is effectively improved and the object points are successfully extracted by these methods. Moreover, the corresponding models are presented for the aligning parameters required. Using the techniques presented in the paper, an inspection system for the wheel-aligning parameters is fulfilled by which the automatic inspection is achieved.
In this paper, the image preprocessing techniques are studied in detail combining with the characteristics of real-time radiography. In view of the characteristics of radiant attenuation, an image processing method is presented to make the relationship between image gray scale and workpiece thickness linear. In order to get better image contrast, an image stretching method based on fuzzy transformation is designed by which the image is effectively enhanced. Meanwhile, another algorithm is also proposed to avoid the complexity of above method. Moreover, the study is carried out to the influence of radiation source focus, which is a very important factor to image quality in real-time radiography. The mathematical model is established for the influence of radiation source focus on real-time radiography image. On the basis of this model, the corresponding algorithm is proposed to eliminate its influence. All these algorithms are studied in detail with corresponding results presented.
This paper introduces the components of the hardware, software, working principle and the defect detection capacity of computer vision system for x-ray real time radiography weld defect detection and evaluation. Meanwhile, the image processing system is studied in which an image transformation algorithm is presented for defect extraction. Moreover, a sectional curve fitting algorithm and an algorithm based on probability are proposed to locate the defects. At the end of the paper, a pattern recognition method is introduced for the automatic identification of weld defects.
KEYWORDS: Control systems, Sensors, Inspection, Data communications, Telecommunications, Optical inspection, Data transmission, Optical sensors, Process control, Switches
In this paper, a distributed control network in autocar-body visual inspection station is presented in which PC is used as the host processor and single-chip microcomputers are employed as slave controllers. The physical interface of the control network and the relevant hardware are introduced in this paper. Meanwhile, a minute research on data communication is performed, relevant protocols on data framing, instruction codes and channel access methods have been laid down and part of related software is presented.
KEYWORDS: Control systems, Sensors, Inspection, Data communications, Optical inspection, Optical sensors, Telecommunications, Data transmission, Process control, Switches
An application of distributed control system in Autocar-body Visual Inspection Station is presented in the paper, a distributed control system using PC as the host processor and single-chip microcomputer as the slave controller is proposed. In this paper, the physical interface of the control network and the relevant hardware are introduced. Meanwhile, a minute research on data communication is performed, relevant protocols on data framing, instruction codes and channel access methods have been laid down and part of related software is presented.
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