The basics for investigating histological brain tissue sections using polarization and differential Mueller matrix microscopy are provided. The structural and logical scheme, design, and examples of results for the Stokes polarimetric mapping technique of microscopic images of deceased brain tissue sections are presented, where orientation (OP) and phase (FP) parameters are determined. Furthermore, the structural and logical scheme, design, and examples of results for the azimuthally-invariant Mueller matrix mapping technique of histological brain tissue sections from deceased individuals are presented, where optical activity (MMI OA) and linear dichroism (MMI LD) maps are determined.
The presented results are from the multifractal analysis of the polarization-correlation maps of the modulus |𝑆𝐾412|(𝑚 × 𝑛) of the fourth parameter of the two-point Stokes vector in microscopic images of histological sections of biological tissues with fibrous (skin dermis) and parenchymal (spleen) architectural structures of the polycrystalline component.
The article contains the results of experimental testing of methods of azimuthal-invariant Mueller-matrix microscopy (Mueller-matrix invariants - MMI) of optically anisotropic fluorophores of samples of histological sections of the brain, liver and kidney, as well as myocardium and lung tissue; temporal detection of variations in the magnitude of the statistical moments of the 1st - 4th orders, characterizing the distributions of the MMI value of linear birefringence and optical activity of samples of histological sections of the brain, liver and kidney, as well as myocardium and lung tissue with different age of damage; determination of the diagnostic efficiency (time interval and accuracy) of establishing the age of damage to human internal organs by digital histological methods of MMI mapping of optical anisotropy of fluorophores in histological sections of the brain, liver and kidney, as well as myocardium and lung tissue.
The paper presents the results of the possibility of a polarization-interference approach to the analysis of microscopic images polycrystalline blood films of patients of benign and malignant prostate tumours with different degrees of differentiation. Measurements and analysis of maps and histograms of the distribution of the local contrast value of polarization-interference distributions of microscopic images of polycrystalline blood films of patients . Determination of the relationship between the statistical moments of the 1st - 4th orders characterizing the distributions of the local contrast value of the polarization-interference distributions of microscopic images of polycrystalline blood films of patients. Determination of statistical criteria for polarization-interference diagnosis of histological sections of biopsy of adenoma and adenocarcinoma with varying degrees of differentiation. Determination of sensitivity, specificity, accuracy of the polarization interferometry method for differential diagnosis of polycrystalline blood films of patients of adenoma and adenocarcinoma with varying degrees of differentiation.
This manuscript presents materials on the study of the spectral dependences of the parameters of the Stokes vector of electromagnetic radiation transmitted through layers of synovial fluid of the knee joint with various types of pathology.The spectral coefficients of Gram - Charlier decomposition of spectral Stokes - polarimetric dependences are established and the sensitivity and specificity of this method for the diagnosis or differentiation of the type of knee joint pathology are determined.
This article contains: structural-logical scheme and analytical description of the differential diagnosis of aseptic and septic loosening of the artificial hip joint endoprosthesis using methods of spectral-selective laser autofluorescent microscopy of maps of coordinate distributions (MIF) and correlation (MCF) of fluorescence intensity of polycrystalline synovial fluid (SF) films; The results of a statistical analysis of the distributions of the MIF and MCF of polycrystalline films of SF patients from the control group and groups with different severity of the hip joint pathology; Results of establishing the strength of the method of spectral-selective laser autofluorescence microscopy of maps of coordinate distributions (MIF) and correlation (MCF) of the fluorescence intensity of polycrystalline films of SF hip joint.
A new analytic parameter-the degree of local depolarization (LPD) of the laser field-is theoretically justified. The technique of experimental measurement of LPD maps is presented. Sets of diagnostic parameters-statistical moments of the first-fourth order, the correlation area and the variance of the logarithmic distribution of the LPD power spectra are obtained. Differential diagnostics of rat skin carcinoma was realized.
The possibility of solving the inverse problem - extraction of information about linear and circular birefringence and dichroism of light scattering biological layers is considered. An analytical model of the optical anisotropy of depolarizing biological tissues is proposed. The Mueller matrix is represented as a superposition of differential matrices of the first and second orders. Interrelations between the parameters of phase and amplitude anisotropy and elements of a first-order differential matrix are obtained. The algorithms for the experimental measurement of the coordinate distributions of the elements of the polarization component of the Mueller matrix of depolarizing biological tissue are found. The symmetry and features of first-order differential matrices of fibrillar (muscle) and parenchymal (liver) depolarizing biological tissues are investigated. The interrelations between the statistical moments of the first and fourth orders and the features of the morphological polycrystalline structure of the biological tissues of various human organs are found. The ways of application of the differential Mueller-matrix mapping method in clinical diagnostics of the distribution of the phase and amplitude anisotropy distributions are proposed.
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