The identification of pesticide and 6-benzylaminopurine (6-BAP) plant growth regulator was carried out using a label-free
opto-fluidic ring resonator (OFRR) biosensor. The OFRR sensing platform is a recent advancement in opto-fluidic
technology that integrates photonic sensing technology with microfluidics. It features quick detection time, small sample
volume, accurate quantitative and kinetic results. The most predominant advantage of the OFRR integrated with
microfluidics is that we can potentially realize the multi-channel and portable biosensor that detects numerous analytes
simultaneously. Antisera for immunoassay were raised in rabbits against the 6-BAP-BSA conjugate. Using the
immunization protocol and unknown cytokinin reacting with same antibody, comparable sensitivity and specificity were
obtained. 6-BAP antibody was routinely used for cytokinin analysis. A sensitive and simple OFRR method with a good
linear relationship was developed for the determination of 6-BAP. The detection limit was also examined. The biosensor
demonstrated excellent reproducibility when periodically exposed to 6-BAP.
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