Organic photoelectric materials have showing a wide application prospect in the fields of energy, environment, information and biology because of their unique advantages. However, it is still not clear for us to understand some basic photo-physical processes (i.e. energy transfer, charge transfer, charge separation and recombination, etc.), which has affected the performance of materials and devices. This is very incompatible with the rapidly growing demand of the organic photoelectric materials and devices, and it has been one of the core problems that constraints the further applications of organic photoelectric materials and devices. The lack of the necessary systems and means is a major reason. Thus, it is a very necessary urgent task to develop new methods and technologies to evaluate the photo-physical properties of organic photoelectric materials and equipment systems. In this paper, an on-line research system for photo-physical properties is established to detect the intrinsic character of the organic photoelectric materials and devices, which integrates the fabrication instrument of the film materials and devices with the online measuring equipment combing with the high vacuum technology and the steady state transient spectrum measurement. A standard OLED device was fabricated and the electrluminescence spectra, current density, brightness, current efficiency and the power efficiency were got using this system avoiding the affect of the air and water. The results indict this system not only plays an important promoting role for the theoretical research of organic photoelectric materials and devices, but also helps improving the research level of organic photoelectric materials and devices. This work is expected to produce some potential innovating results with the international advanced level and make contributions to needs of national strategy.
Hg2+ ions are one of the most toxic heavy metal ion pollutants, and are caustic and carcinogenic materials with high cellular toxicity. The Hg2+ ions can accumulate in the human body through the food chain and cause serious and permanent damage to the brain with both acute and chronic toxicity. According to the US Environment Protection Agency (EPA) guidelines, Hg2+ ions must be at concentrations below 1 ng/ml (10 nM) in drinking water. If the Hg2+ ions are higher than 2.5 ng/ml in serum, that will bring mercury poisoning. The traditional testing for Hg2+ ions includes atomic absorption, atomic fluorescence, and inductively coupled plasma mass spectrometry. These methods are usually coupled with gas chromatography, high-performance liquid chromatography, and capillary electrophoresis. However, these instrument-based techniques are rather complicated, time-consuming, costly, and unsuitable for online and portable use. An ultrasensitive and selective detection of mercury (II) in serum was investigated using a laser scanning confocal imaging-surface plasmon resonance system (LSCI-SPR). The detection limit was as low as 0.01 ng/ml for Hg2+ ions in fetal calf serum and that is lower than that was required Hg2+ ions must be at concentrations below 1 ng/ml by the US Environment Protection Agency (EPA) guidelines. This sensor was designed on a T-rich, single-stranded DNA (ssDNA)-modified gold film, which can be individually manipulated using specific T–Hg2+–T complex formation. The quenching intensity of the fluorescence images for rhodamine-labeled ssDNA fitted well with the changes in SPR. The changes varied with the Hg2+ ion concentration, which is unaffected by the presence of other metal ions. A good liner relation was got with the coefficients of 0.9116 in 30% fetal calf serums with the linear part over a range of 0.01 ng/ml to10 ng/ml.
The laser scanning confocal microscope (LSCM) offers several advantages over conventional optical microscopy, but
most LSCM work is qualitative analysis and it is very hard to achieve quantitative detection directly with the changing of
the fluorescent intensity. A new real time sensor system for the antibody-antigen interaction detection was built
integrating with a LSCM and a wavelength-dependent surface plasmon resonance (SPR) sensor. The system was applied
to detect the bonding process of human IgG and fluorescent-labeled affinity purified antibody in real time. The
fluorescence images changing is well with that of SPR wavelengths in real time, and the trend of the resonance
wavelength shift with the concentrations of antibody is similar to that of the fluorescent intensity changing. The results
show that SPR makes up the short of quantificational analysis with LSCM with the high spatial resolution. The sensor
system shows the merits of the of the LSCM and SPR synergetic application, which are of great importance for practical
application in biosensor and life science for interesting local interaction.
Surface plasmon resonance (SPR) sensor has been widely investigated as an exceedingly powerful tool for biological and
chemical detection in the past decay. As an essential component of both academic and commercial laboratories, several
methods have been employed to monitor the excitation of SPR by measuring the light reflected from the sensor interface
including modulation of angle, wavelength, intensity, phase and polarization. Among these methods, angle modulation
(AM) and wavelength modulation (WM) are the most widely adopted in practice. However, the normal sensors system of
the angle modulation was built by two ratable stages with a flow cell attaching a prism, and the angle of incidence
rottingθ, the angle of reflect rotting 2θ. The mechanical structure can only be used in the angle modulation SPR sensor.
In the present study, a new SPR is designed by a mechanical structure which can transform freely from wavelength to
angle modulation. The different concentration Ethanol solution is tested by this system, and the linear range is very well
from the volume ratio 0% to 60%. The primary application is done in real time detection of human IgG and antibody
interaction, and the experimental results indicate that the new SPR system can detect human antibody at low
concentration 1x10-11g/ml with highly sensitivity and flexibility. The new SPR system is of great importance for
potentially practical application in biosensor and life science with the extended the functions of the conventional SPR
sensor, and provides the real-time and in-situ detection of the biomolecules.
Confocal microscopy offers several advantages over conventional optical microscopy; we show an experimental
investigation laser scanning confocal microscope as a tool to be used in cubic boron nitride (cBN) film-based fluorescent
sensor research. Cubic boron nitride cBN film sensors are modified with dansyl chloride and rhodamine B isothiocyanate
respectively. Fluorescent modification quality on the cubic boron nitride film is clearly express and the sensor ability to
Hg2+ cations and pH are investigated in detail. We evidence the rhodamine B isothiocyanate modified quality on cBN
surface is much better than that of dansyl chloride. And laser scanning confocal microscope has potential application
lighttight fundus film fluorescent sensor research.
The noninvasive measurement of human blood glucose has been a hotspot for a long time to all clinical workers over the world. In this paper the noninvasive measurement of human blood glucose with diffusion reflectance NIR spectrum method is presented. The thumb fingertip, the palm and the wrist with vein are chosen for the collection of diffusion reflectance NIR spectroscopy from six different age healthy volunteers using Nexus-870 and its NIR fiber port smart accessory. The calibration model is set up in 7500~8500cm-1 region that has the absorption of the glucose using Partial Least Squares (PLS) method with the first and second derivative spectral that had been smoothed and baseline corrected for single volunteer. The actual blood glucose value is determined by an ultraviolet spectrophotometer. The model with the spectrum obtained at the wrist is better than from other part for all volunteers, and it is much steadier with the second derivative pretreatment spectral than with the first derivative ones. The correlative coefficients are all over 0.93772; RMSECs are all less than 0.310 and the max differences are between -0.6mmo/L and +0.8mmo/L with the second derivative method. Some samples are kept for prediction with their own model. The differences are under 0.875529mmol/L.
KEYWORDS: Glucose, Blood, Near infrared, Calibration, Near infrared spectroscopy, Spectroscopy, Absorption, Signal to noise ratio, Chemometrics, Principal component analysis
Principal Component Regression (PCR), Partial Least Square (PLS) and Artificial Neural Networks (ANN) methods are used in the analysis for the near infrared (NIR) spectra of glucose in the whole blood. The calibration model is built up in the spectrum band where there are the glucose has much more spectral absorption than the water, fat, and protein with these methods and the correlation coefficients of the model are showed in this paper. Comparing these results, a suitable method to analyze the glucose NIR spectrum in the whole blood is found.
Near-infrared spectroscopy is a fairly powerful recent but well-established tool that can be used to study and measure biological and chemical concentrations, and the water is an important portion of organism and chemical reagent. Near-infrared (NIR) spectroscopic studies of water have long been a subject of keen interest from various points-of-view. The importance of the NIR spectrum of water stems from the fact that the frequencies and intensities of bands due to water alter with changes in the strength of hydrogen bonds and hydration. The NIR absorbance spectroscopy of water and the single-beam of the water were measured over a temperature range of 10-80°C at increments of 5°C and a spectroscopic range of 1-2.5μm. It had been validated that positions of water absorption bands centered at 6900cm-1 depend heavily on temperature effects. Temperatures can also influence the bands of the water molecules combinations and overtones. The trend of water absorbance spectroscopy's changing is discussed detailed in this paper. Especially the variety of the band amplitude and the shift tendency in peak position are all presented in this paper. At the same time, the spectroscopies that are collected with different parameter setup of the spectrograph during the temperature change are obtained. Partial least squares (PLS) calibration models were constructed at sixteen separate temperatures.
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