We present our new microscope prototype, which uses the single-pixel imaging method to image fluorescent samples. Specifically, our microscope generates sinusoidal patterns of excitation light by interfering two laser beams ‘steered’ individually by optical deflectors. We imprint a series of such sinusoidal patterns onto the sample and use a single-pixel detector to acquire a signal-readout for each pattern. Ultimately, this allows us to ‘build’ an image of the sample. Our application of optical deflectors opens the possibility to implement a microscope that provides sharp images fast while optimizing the fluorescence duty cycle, making it suitable for long-term observation of organ-on-chip systems.
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