In recent decades, gold nanorods have been widely used in biomedical fields, such as drug delivery, phototherapy, diagnosis and biosensing, due to their unique properties. And biosafety assessment and therapeutic effect of gold nanorods are very important for their application in biomedicine. In order to evaluate the biological safety and therapeutic effect of gold nanorods, it is necessary to solve the problem of the interaction between cells and gold nanorods. However, the complexity of the gold nanorods properties, cell type and microenvironment will affect the interaction between cells and gold nanorods. In this study, by comparing normal ovarian cancer cells (HOSEpiC) with cancerous ovarian cancer cells (HO-8910), we investigated the toxicity of gold nanorods to the two different kinds of cells, the uptake of gold nanorods by the two different kinds of cells and the intracellular distribution of gold nanorods after they enter the cells. In addition, the effect of protein corona on the interaction of gold nanorods with ovarian cells was also explored. The results showed that the cytotoxicity of gold nanorods and the uptake of gold nanorods by cells were affected by many factors. And the presence of protein corona significantly reduced the internalization ability of gold nanorods. This study will provide a research basis for the future application of gold nanorods in the diagnosis and treatment of ovarian cancer.
Gold nanorods have special optical properties, good biological compatibility and easy preparation, making them have broad application prospects in drug carriers and medical imaging. In this paper, the two photon luminescence of silica modified gold nanorods incubated with different subtypes of breast cancer cells was observed. We demonstrate the effects of different subtypes breast cancer cells on cellular uptake of gold nanorods. And The results show that significant decreases internalization of gold nanorods with the presence of fetal bovine serum protein in cell culture. Two-photon imaging revealed that cellular uptake of gold nanorods was affected by serum and subtypes of cells, and movement of gold nanorods in the cells was observed. Our findings can provide useful information for medical imaging of gold nanorods in biomedical application.
Gold nanorods (GNRs) are promising nanomaterials for applications in biomedicine because of their special optical properties, and tremendous works have reported their potential in imaging, diagnosis and treatment of cancer. Unfortunately, study on gold nanorods and cell interactions is still incomplete, and the interplay between gold nanorods and different subtype of breast cancer cells is rarely reported. In the study, two different type of gold nanorods (GNRs and GNRs@SiO2) was synthesized. And we investigated the interactions of gold nanorods (GNRs and GNRs@SiO2) with ER+ (MCF-7)/ER-(MDA-MB-231) breast cancer cells, including cytotoxicity, cellular uptake. Our results showed that GNRs are more cytotoxic to MCF-7 and MDA-MB-231 cells than GNRs@SiO2. And MCF-7 and MDA-MB-231 cells internalize GNRs in a time dependence, and MCF-7 is far more effective in taking up GNRs. The result suggests different subtype of tumor cells should be considered to fully understand the interactions of gold nanorods and cells.
Imaging brain circuits is the basis for us to understand brain function and dysfunction. However, imaging axon at micrometer resolution while tracing the centimeter-scale axon projection across the whole-brain is still challenging. Here, we developed a fluorescence micro-optical sectioning tomography (fMOST) imaging system based on confocal fluorescence imaging scheme that can obtain whole brain image stack for visualizing brain circuits at neurite level. We use confocal detection to remove fluorescence background to clearly see one single neurite and use acoustical optical deflector (AOD), an inertia-free beam scanner to realize fast and prolonged stable imaging. We had acquired several complete datasets of whole-mouse brain at a one-micron voxel resolution. Based on these datasets, the uninterrupted tracing of brain-wide, long-distance axonal projections was demonstrated for the first time using a systematic reconstruction and annotation pipeline. Our method is believed to open an avenue to exploring both local and long-distance neural circuits that are related to brain functions and brain diseases down to the neurite level.
A recently reported micro-optical sectioning tomography system has great potential to draw the neuronal circuits of large brain volume with submicron resolution by combining fine mechanic sectioning with simultaneous optical imaging. However, sectioning the fluorescence sample sometimes induces tears between adjacent tiles and causes difficulties in continuous fiber tracing from fluorescence imaging. A confocal detection to recover the interruptions of the nerve fiber is introduced. With a 50-μm-width confocal slit, the signal-to-background ratio is increased 16- to 49-fold more than that without the slit, which effectively improves the detectability of the signal in the interruptions and enables continuous tracing of the neuronal circuits.
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