Ms. Jinfeng Ning Profile

Jinfeng Ning

at Tulane Univ

SPIE Involvement:

Author

Publications (2)

Proceedings Article | 29 March 2007 Paper

Retinal oxygen saturation evaluation by multi-spectral fundus imaging

Bahram Khoobehi, Jinfeng Ning, Elise Puissegur, Kimberly Bordeaux, Madhusudhanan Balasubramanian, James Beach

Proceedings Volume 6511, 65110B (2007) https://doi.org/10.1117/12.710030

KEYWORDS: Oxygen, Retina, Arteries, Tissues, Veins, Cameras, Eye, Image registration, Blood, Reflectivity

Read Abstract +

Purpose: To develop a multi-spectral method to measure oxygen saturation of the retina in the human eye. Methods: Five Cynomolgus monkeys with normal eyes were anesthetized with intramuscular ketamine/xylazine and intravenous pentobarbital. Multi-spectral fundus imaging was performed in five monkeys with a commercial fundus camera equipped with a liquid crystal tuned filter in the illumination light path and a 16-bit digital camera. Recording parameters were controlled with software written specifically for the application. Seven images at successively longer oxygen-sensing wavelengths were recorded within 4 seconds. Individual images for each wavelength were captured in less than 100 msec of flash illumination. Slightly misaligned images of separate wavelengths due to slight eye motion were registered and corrected by translational and rotational image registration prior to analysis. Numerical values of relative oxygen saturation of retinal arteries and veins and the underlying tissue in between the artery/vein pairs were evaluated by an algorithm previously described, but which is now corrected for blood volume from averaged pixels (n > 1000). Color saturation maps were constructed by applying the algorithm at each image pixel using a Matlab script. Results: Both the numerical values of relative oxygen saturation and the saturation maps correspond to the physiological condition, that is, in a normal retina, the artery is more saturated than the tissue and the tissue is more saturated than the vein. With the multi-spectral fundus camera and proper registration of the multi-wavelength images, we were able to determine oxygen saturation in the primate retinal structures on a tolerable time scale which is applicable to human subjects. Conclusions: Seven wavelength multi-spectral imagery can be used to measure oxygen saturation in retinal artery, vein, and tissue (microcirculation). This technique is safe and can be used to monitor oxygen uptake in humans. This work is original and is not under consideration for publication elsewhere.

SPIE Journal Paper | 1 September 2005 Open Access

Algorithms for automated oximetry along the retinal vascular tree from dual-wavelength fundus images

Harihar Narasimha-Iyer, James Beach, Bahram Khoobehi, Jinfeng Ning, Hiroyuki Kawano, Badrinath Roysam

JBO, Vol. 10, Issue 05, 054013, (September 2005) https://doi.org/10.1117/12.10.1117/1.2113187

KEYWORDS: Image segmentation, Oxygen, Arteries, Veins, Oximetry, Image processing algorithms and systems, Blood, Algorithm development, Retina, Reconstruction algorithms

Read Abstract +

DOWNLOAD PAPER

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Keywords/Phrases

Search In:

Publication Years