In a broadband coherent anti-Stokes Raman scattering (CARS) microspectroscopy with supercontinuum (SC),
the simultaneously detectable spectral range is limited by the spectral continuity and simultaneity of various
spectral components of SC in an enough bandwidth. According to our theoretical analysis and experiments, the
optimal experimental conditions are obtained. The broadband time-resolved CARS microspectroscopy based on
the SC with required temporal and spectral distributions is achieved. The global CARS spectrum with well
suppressed nonresonant background noise can be obtained in a single measurement and used as the imaging
contrast. It will be more helpful to provide a complete and accurate molecular atlas, and to exhibit a potential to
understand not only both the solvent dynamics and the solute-solvent interactions, but also the mechanisms of
chemical reactions in the fields of biology, chemistry and material science.
We present a novel fluorescence sectioning endoscopy that is based on a fiber-bundle and using speckle pattern
illumination. The laser speckle pattern is produced by a rotating diffuser and delivered into the fiber bundle for whole-field
excitation of the sample. The fluorescence is collected and transmitted to a CCD camera via the endoscope optics
and fiber bundle. A sequence of fluorescent images are acquired and processed to reconstruct a 2D depth-resolved image
of the sample. The fiber-bundle based endoscopy has similar sectioning capability to that of a laser scanning confocal
microscopy but without scanning. Its other advantages include compactness and low cost, which makes it potentially
viable for implementation in a portable clinical system.
In this paper, we present a novel wide-field fluorescence sectioning microscopy using dynamic speckle illumination that
provides depth discrimination. Some simple modifications are made to a conventional wide field fluorescence
microscope, which allows for the illumination of the sample using dynamic speckle patterns and the acquisition of a
sequence of independent wide-field fluorescence images. The full-field fluorescence image of the sample at a particular
depth is obtained using specific digital image processing algorithms. Compared with conventional confocal scanning
fluorescence microscopy, this novel imaging modality is capable of providing a reasonably high sectioning performance
with a compact and cost-effective system. The experimental results show that the wide-field fluorescence sectioning
microscopy can provide high contrast imaging even in scattering biological media, therefore has wide potential
applications in biomedical research and clinics.
From the application point of view of CIM or CAD/CAPP/CAM, there should be a unified part model through which the different application system can share the part information. Using feature as the basic information unit, form feature and its binary tree as the skeleton, a new practical part model regarding the rotational components is presented in this paper. The data structure of the model is discussed in detail. An application example of the model is shown in this article. The model has been found to be flexible and effective in part creation, representation, automatic process planning, and CAM.
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