Raman-Trapping-Microscopy integrates Nobel prize technologies--Raman and Optical Trapping--into a microscope making it available for biomedical use. Cells, bacteria, exosomes or viruses are analyzed reflecting their natural features and behavior.
Raman spectroscopy increasingly becomes a valuable analytical tool in biomedicine. A novel Raman microscope designed for biomedical applications was used to discriminate viability states and cell types of Hodgkin’s disease as well as different neural and invading glioblastoma cells within a human engineered neural tissue (ENT).
A UV-laser microbeam was successfully used to induce fusion of early embryonic cells. The developmental capacity of the laser-fused cells was examined using in vitro culture methods. Blastomeres within mouse two-cell embryos were fused with 3 - 10 subsequent laser pulses in order to produce tetraploid embryos. Thirty-one percent of the laser treated embryos fused and 10% of those developed to the morula or blastocyst stage. With 1 - 10 successive laser pulses cattle oocytes were fused with cytoplasts. Thirty-six percent of the laser treated cells fused and 10% of those cleaved to the 6- and 8-cell stage. These preliminary results indicate that a UV- laser microbeam combined with an optical tweezers may facilitate the manipulation of embryonic cells and can be a helpful tool in polyploidy studies and in cytoplasmic transfer experiments.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.