Using a photon-counting quantum light spectroscopy that probes photosynthetic light harvesting with a single photon at a time, we experimentally demonstrated that photosynthesis begins and proceeds with a single quantum of energy. We report the observation of individual single-photon absorption and emission events in spatially distinct regions in photosynthetic systems. The experiments were carried out on an ensemble of pigment-protein complexes light-harvesting 2 from purple bacteria Rhodobacter sphaeroides under ambient conditions in vitro.
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