Using rhodamine 6G, hydrazine hydrate, and 9-anthracene as raw materials, a ratio-based fluorescent probe compound RHA based on rhodamine-anthralaldehyde derivatives was successfully synthesized. The structure of the probe compound was characterized by 1H NMR. The selectivity, anti-interference, detection limit and response time of the probe compound to metal ions were studied by fluorescence spectroscopy and colorimetry. The results show that the probe compound can selectively recognize Hg2+, its color change can be seen with the naked eye, and has certain anti-interference. The detection limit is 5.27×10-6 mol·L-1. In addition, the response of the probe compound to Hg2+ is also very fast, and it can react completely in 90 minutes.
The determination of selenocysteine was investigated based on the fact that the -SeH bond in selenols can specifically undergo nucleophilic addition reaction with the Se-N bond in benzoselenadiazole. The results obtained showed that the pH of t determination system was 4.7 and the optimum temperature was 37°C. The linear relationship was F=90.246c+298.36, and the correlation coefficient R2 was 0.9916. The relative error of this method was less than 2.2%, and the recoveries were in the range of 96.6%~103%. This experimental method is based on the nucleophilic addition reaction of -SeH, which enables the rapid and accurate determination of selenocysteine without catalysis, heating and long waiting time. This approach provides some reference for the study of the physiological significance of selenium.
As an important restoration substance in cells, reduced glutathione (GSH) plays an important physiological role in the human body. In this paper, GSH was determined by indirect Spectrophotometry. Through the optimization experimental conditions, the amount of Fe3+-1,10-phenanthroline chromogenic concentration was 0.8 mg/mL, and the optimum pH value was 4.0 mL, and the amount of buffer solution was 5.0 mL, and the heating time and cooling time were 10 min. In addition, the absorbance was measured at 510 nm, and GSH was in the range of 0~0.4 mg/mL. The linear regression equation was A=1.7981c+0.1348 (which A represents the absorbance and c represents the concentration of GSH), and the correlation coefficient was r2 = 0.9955. The method was applied to the determination of reduced GSH in three GSH samples. The indirect spectrophotometry method is simple, low cost and easy to operate.
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