Leaf area index (LAI) is an important phenotypic trait closely related to plant vigor and biomass. It is also a key parameter used in crop growth modeling. However, manually measuring LAI in the field can be slow and labor intensive. High resolution remote sensing, such as unmanned aircraft systems (UAS), has been explored for LAI estimation but with limited data sources, usually RGB and multispectral imagery. As UAS-based thermal infrared (TIR) imaging becoming readily available in agriculture, it is worth investigating the potential of its role in improving LAI estimation. In this study we evaluated the importance of canopy temperature measured by UAS-based TIR and multispectral imagery on maize LAI quantification within a breeding context (23 genotypes). Five plot-level features (canopy temperature, structure and two common vegetation indices) were extracted from the images, and used as inputs of machine learning models for the LAI estimation. The performance of the estimation was evaluated with a 5-fold cross validation with 30 random repeats for 162 samples. Results showed that, canopy temperature, together with canopy structure as model predictors, slightly improved LAI estimation (root mean square error, RMSE of 0.853 m2/m2 and coefficient of determination, R2 of 0.740) than those models without temperature difference (RMSE of 0.917 m2/m2 and R2 of 0.706) for the various genotypes included in this study. In addition, canopy temperature showed moderate and more stable significance in estimating LAI than plant height and image uniformity. Its contribution to the estimation was comparable or even higher than those from vegetation indices when being modeled with random forest in this study. These relationships may be changed with a single or less genotypes which can be explored in future studies.
Leaf stomata regulate the process of gas exchange between the plant and the atmosphere, therefore play an important role in plant growth and water use. Thermal infrared sensing of leaf surface temperature is proved to be an indirect but effective approach to estimate leaf stomatal conductance, and shows the potential to rapidly differentiate genotypes for water-use related traits. The objective of this study was to estimate leaf stomatal conductance from thermal IR images of crops and relevant environmental parameters. The experiment was conducted in the NU-Spidercam field phenotyping facility near Mead, NE. Leaf stomatal conductance was measured from soybean, sorghum, maize, and sunflower using a leaf porometer. Thermal IR images of the crop canopies were captured by a thermal IR camera and then processed to extract crop canopy temperature (Tc). In addition, weather variables including solar radiation, air temperature, relative humidity, and wind speed were extracted from a nearby weather station. Correlation analysis was implemented to explore the relationships between these variables. Multiple linear regression (MLR), random forest (RF), gradient boosting machine (GBM) were applied to model stomatal conductance from Tc and weather variables. The Pearson correlation coefficients between predicted and measured stomatal conductance were 0.495 for MLR, 0.591 for RF, and 0.878 for GBM when Tc was not used as an input variable. After adding Tc as input, Pearson correlation coefficients were improved to 0.584 for MLR, 0.593 for RF, and 0.896 for GBM. The mean absolute errors for the three models were 225, 237, and 129 mmol/(m2·s) when Tc was included as a model input. This research would lead to rapid assessment of leaf stomatal conductance and crop water status using thermal IR imaging.
Though unmanned aircraft systems (UAS) are widely used in agriculture, their current positioning accuracy in a radius of 0.5 to 2 meters is still too low to pinpoint a crop row or to precisely overlay temporal multi-source field maps together without a valid geometric calibration. The positioning accuracy of UAS deployed with real time kinematic (RTK) global navigation satellite system (GNSS) can be largely increased to a centimeter level, which was claimed from the manufacturers. This paper includes the preliminary test results of positioning accuracy of a commercial RTK UAS over a set of fixed position panels in our customized scenarios. Images were collected in three GNSS modes (regular GNSS without RTK, RTK mode 1 - not corrected by the positioning error of the base station, and RTK mode 2 - corrected by the positioning error of the base station) in static and in-flight settings. In the static setting, horizontal accuracies were 2.17 cm for the RTK mode 2, 12.11 cm for the RTK mode 1, and 11.46 cm for the regular GNSS mode. The significant result of horizontal accuracy in the in-flight setting was that RTK mode 2 without GCPs (2.82 cm) showed comparable accuracy with the commonly used regular GNSS mode with GCPs (1.34 cm). The vertical positioning accuracy in the static setting were 6.01 cm for the RTK mode 2, 5.65 cm for the RTK mode 1, and 10.48 cm for the regular GNSS mode. The accuracy of height measurement from digital surface models (DSMs) without and with GCPs in RTK mode 2 were 4.81 cm and 3.72 cm, respectively, which were the best performance among the three modes. In summary, the RTK UAS tested in this study showed great potential in eliminating the requirement of using GCPs and in high-positioning-accuracy application. The next phase is to test the system in field for accurate crop height measurement at different growth stages in agricultural application.
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