Metabolic features of mitosis remain poorly understood because this phase of the cell cycle is rapid and heterogeneous between cells within a dish. Label-free optical metabolic imaging (OMI) can monitor rapid changes in cell metabolism with single cell resolution using two-photon microscopy of the optical redox ratio (NAD(P)H/FAD) and NAD(P)H fluorescence lifetimes. Here, we brought together image analysis tools to quantify OMI time-courses of single cells undergoing mitosis across multiple cell lines. Alignment of OMI and morphological features over time provided unique insight into metabolic changes during mitosis within unperturbed systems.
The cell cycle is extensively characterized, yet there is much to learn about the decision-making process involved in cell division. Here, the optical redox ratio (ratio of NADH to FAD fluorescence intensity) of MCF10A cells was imaged every 20 minutes over 12 hours using multiphoton microscopy. Cell tracking was used to monitor individual cells over time. We found a positive correlation in the variations of the optical redox ratio with the phases of the entire cell cycle. This study reveals the novel role of redox signaling in the progression the cell cycle.
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