Stimulated Raman Histology (SRH) can be used for label-free histological tissue analysis. However the current approaches are limited to two Raman peaks, thus restricting chemically-specific insights. We present a broadband coherent Raman platform (CRP), which overcomes these limitations combining an all-fiber dual output laser and a multichannel lock-in amplifier. This enables the simultaneous detection of 38 channels across the entire CH spectrum (2800-3100 cm-1) in parallel, facilitating chemometric and multimodal tissue analysis. By incorporating AI-driven virtual H&E staining and tissue segmentation for diagnostic purposes, it advances SRH towards clinical-diagnostic.
We combine an all-fiber dual wavelength, self-synchronized laser and a dedicated multi-channel detection unit to perform state-of-the-art multiplex Stimulated Raman Scattering (SRS) microscopy. The system covers the full CH spectrum in 1 μs reaching shot-noise limited performances with 25 μW per detection channel. This all-inone solution is based on a passively synchronized dual-wavelength laser source with shot-noise limited relative intensity noise from 600 kHz and a modular multi-channel lock-in detection unit. The synergistic design between laser source and detection system simplifies multiplex SRS implementation for real-time full-chemical imaging.
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