Wigner distribution function (WDF) is a complete representation of the optical field with arbitrary coherent state and can be regarded as a local spectrum. It has unique advantages for representation, measurement and manipulation of partially coherent fields. Image sensors can only obtain the two-dimensional intensity data, which is the shearing projection of WDF. The shearing of WDF, which equals to the propagation of optical field, embodies the coherent properties of optical field. In this paper, a phase space retrieval method based on three-dimensional (3D) intensity focus stack combined with an iterative optimization is proposed. WDF with arbitrary coherent state can be reconstructed by 3D intensity sequences, which can realize the local spectrum retrieval and the analysis of spatial partially coherent fields.
We present a 3D label-free refractive index (RI) imaging technique based on single-exposure intensity diffraction tomography (sIDT) utilizing a color-multiplexed illumination scheme. In our method, the chromatic LEDs corresponding R/G/B channels in an annular programmable array provide oblique illumination geometry which matches the numerical aperture of the objective precisely to maximize the spectrum coverage. A color intensity image encoding the scattering field of the specimen from different directions is captured, and monochromatic intensity images with respect to three color channels were separated and then used to recover the 3D RI distribution of the object following the process of IDT. In addition, the axial chromatic dispersion of focal lengths at different wavelengths introduced by the chromatic aberration of the objective lens and the spatial position misalignment of the ring LED source in the imaging system’s transfer functions modeling are both corrected to significantly reduce the artifacts in slice-based deconvolution procedure for the reconstruction of 3D RI distribution. Experimental results on MCF-7, Spirulina algae, and live C. elegans samples demonstrate the solid performance of the sIDT method in label-free, high-throughput, and real-time (∼ 24 fps) 3D volumetric biological imaging applications.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.